FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395734
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA386789.773736904082035No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA223915.658093567901671No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA213805.4026189308980275No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA195124.9305846856727No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA169944.29429869558845No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA139853.5339394643876947No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA135533.4247752278045356No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA134243.392177573824842No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA123263.114718472509312No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60161.5202130724173308No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG42951.0853249910293277No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG32730.8270707091126869No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25860.6534692495464125No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG25560.6458883997836931No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25140.635275210115886No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG24120.60950032092264No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22470.5678056472276832No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19260.4866905547665857No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG18960.4791097050038663No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG18950.4788570100117756No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG17340.4381731162851814No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG17130.4328665214512779No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG16650.4207371618309268No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14990.37878979314387945No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG14810.37424128328624784No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14580.36842929846816297No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14450.36514426357098456No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG14400.3638807886105313No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG14300.36135383868962484No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG14080.3557945488636306No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG13580.3431597992590983No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG13510.34139093431446377No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13410.3388639843935573No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG12360.3123310102240394No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG12170.3075298053743171No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12160.30727711038222644No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG11950.3019705155483228No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG11080.2799860512364366No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG11070.2797333562443459No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG10840.27392137142626105No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG10120.2557273319957345No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10030.2534530770669187No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9830.24839917722510574No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG9610.24283988739911153No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG9270.23424825766802954No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9050.22868896784203532No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9010.22767818787367272No Hit
GACTACTAGGGTATCTAATCCTGCAGCCACCCGTAGG8940.2259093229290382No Hit
GACTACCAGGGTATCTAATCCTGCAGCCCCCCGTAGG8730.22060272809513462No Hit
GACTACACGGGTATCTAATCCTGCAGCCCCCCGTAGG8560.2163069132295936No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8500.21479074327704975No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8190.20695719852223968No Hit
GACTACCCGGGTATCTAATCCTGCAGCCCCCCGTAGG8130.2054410285696958No Hit
GACTACAAGGGTATCTAATCCTGCAGCCCCCCGTAGG8060.2036721636250613No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8020.20266138365669867No Hit
GACTACTGGGGTATCTAATCCTGCTGCCCCCCGTAGG7910.19988173874370155No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7910.19988173874370155No Hit
GACTACAGGGGTATCTAATCCTGCAGCCTCCCGTAGG7680.1940697539256167No Hit
GACTACTCGGGTATCTAATCCTGCAGCCGCCCGTAGG7650.19331166894934476No Hit
GACTACTCGGGTATCTAATCCTGCAGCCTCCCGTAGG7410.18724698913916923No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7290.18421464923408148No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCATGCCTCAG7160.18092961433690308No Hit
GACTACAGGGGTATCTAATCCTGCAGCCGCCCGTAGG7130.18017152936063113No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7060.17840266441599661No Hit
GACTACTAGGGTATCTAATCCTGCAGCCTCCCGTAGG7050.17814996942390596No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG7000.17688649446345273No Hit
GACTACCGGGGTATCTAATCCTGCAGCCACCCGTAGG6860.17334876457418366No Hit
GACTACTAGGGTATCTAATCCTGCAGCCGCCCGTAGG6810.17208528961373043No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6540.16526252482728299No Hit
GACTACACGGGTATCTAATCCTGCAGCCACCCGTAGG6170.15591281011992905No Hit
GACTACCAGGGTATCTAATCCTGCAGCCACCCGTAGG6000.15161699525438804No Hit
GACTACCGGGGTATCTAATCCTGCAGCCGCCCGTAGG5920.14959543531766287No Hit
GACTACCCGGGTATCTAATCCTGCAGCCACCCGTAGG5910.14934274032557224No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5900.14909004533348158No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5790.14631040042048446No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5720.14454153547584994No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5620.14201458555494348No Hit
GACTACCGGGGTATCTAATCCTGCAGCCTCCCGTAGG5420.13696068571313053No Hit
GACTACAAGGGTATCTAATCCTGCAGCCACCCGTAGG5400.13645529572894924No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5390.13620260073685858No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5340.13493912577640538No Hit
GACTACACGGGTATCTAATCCTGCAGCCTCCCGTAGG5050.12761097100577662No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5010.12660019103741402No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4900.1238205461244169No Hit
GACTACCAGGGTATCTAATCCTGCAGCCGCCCGTAGG4770.12053551122723849No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4750.1200301212430572No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG4700.11876664628260396No Hit
GACTACTGGGGTATCTAATCCCCGCTTCTGCAGCCCCCCGTAGG4680.11826125629842267No Hit
GACTACAGGGGTATCTAATCCTGCTGCCCCCCGTAGG4660.11775586631424138No Hit
GACTACAAGGGTATCTAATCCTGCAGCCGCCCGTAGG4630.11699778133796945No Hit
GACTACACGGGTATCTAATCCTGCAGCCGCCCGTAGG4500.11371274644079103No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4490.11346005144870039No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCATGCCTCAG4480.11320735645660973No Hit
GACTACCCGGGTATCTAATCCTGCAGCCGCCCGTAGG4470.1129546614645191No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4430.11194388149615651No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4380.11068040653570328No Hit
GACTACCCGGGTATCTAATCCTGCAGCCTCCCGTAGG4370.11042771154361262No Hit
GACTACAAGGGTATCTAATCCTGCAGCCTCCCGTAGG4370.11042771154361262No Hit
GACTACCAGGGTATCTAATCCTGCAGCCTCCCGTAGG4360.11017501655152198No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4350.10992232155943134No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4310.10891154159106875No Hit
GACTACTAGGGTATCTAATCCTGCTGCCCCCCGTAGG4300.10865884659897809No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4300.10865884659897809No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4260.1076480666306155No Hit
GACTACTCGGGTATCTAATCCTGCTGCCCCCCGTAGG4250.10739537163852488No Hit
GACTACTGGGGTATCTAATCCTGCTGCCACCCGTAGG4200.10613189667807163No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4100.10360494675716515No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCATGCCTCAG4090.10335225176507452No Hit
GACTACTGGGGTATCTAATCCTCGCAGCCCCCCGTAGG4010.10133069182834933No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3960.10006721686789612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)