FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253736
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT127395.02057256360942No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT120744.758489138316991No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC120524.749818709209572No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC108624.280827316581013No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT86363.403537535075827No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT83473.289639625437463No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC83353.2849103004697797No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC74172.9231169404420343No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT41781.6465933095816125No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC36951.456237979632374No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT32271.271794305892739No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT29751.1724784815713971No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28331.1165148027871488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27951.1015386070561528No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24370.9604470788536117No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT22800.8985717438597598No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC22270.87768389191916No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC20860.8221143235488855No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20830.8209319923069647No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT19880.7834915029794748No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18710.737380584544566No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC18500.7291042658511209No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC17550.6916637765236309No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17510.6900873348677365No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17330.6829933474162121No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC16400.6463410789166693No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC16330.6435823060188542No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGTCAATGGGTGAGAGCCTGAA14290.5631837815682441No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14160.5580603461865876No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT14080.554907462874799No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC13580.53520194217612No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC13380.5273197338966484No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT13140.5178610839612826No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12070.47569126966610964No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12060.4752971592521361No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGTCAATGGGTGAGAGCCTGAA11800.465050288488823No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC11370.4481035406879591No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC10710.42209225336570294No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT9490.3740107828609263No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT9220.3633698016836397No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9090.35824636630198314No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGTCAATGGGTGAGAGCCTGAA9000.354699372576221No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT8460.3334174102216477No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGTCAATGGGTGAGAGCCTGAA8430.332235078979727No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC8190.32277642904436105No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8000.31528833117886307No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7230.2849418293028975No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7200.2837594980609768No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7090.2794242835072674No Hit
CCTACGGGGGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG6900.2719361856417694No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6780.26720686067408644No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6550.25814232115269414No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6500.25617176908282624No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT6200.24434845666361887No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC6100.24040735252388307No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC5950.23449569631427944No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG5870.23134281300249077No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5440.2143960652016269No Hit
CCTACGGGTGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG5370.2116372923038118No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5360.21124318188983823No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5320.20966674023394394No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5210.20533152568023458No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5000.1970552069867894No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC4970.1958728757448687No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC4770.1879906674653971No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4690.18483778415360846No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT4680.18444367373963488No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4670.1840495633256613No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4670.1840495633256613No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4600.18129079042784627No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC4460.17577324463221616No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC4430.17459091339029542No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC4380.17262036132042752No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC4290.16907336759466532No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT4280.16867925718069174No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4230.16670870511082386No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4100.1615852697291673No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4050.15961471765929944No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT3980.15685594476148437No Hit
CCTACGGGAGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG3940.15527950310559005No Hit
CCTACGGGCGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG3920.15449128227764292No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC3890.15330895103572217No Hit
CCTACGGGGGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC3660.14424441151432985No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGAGTAGTC3580.14109152820254123No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT3480.13715042406280542No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCTGTAGTC3450.1359680928208847No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT3360.13242109909512248No Hit
CCTACGGGTGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC3360.13242109909512248No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3340.13163287826717532No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3300.13005643661128102No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3270.12887410536936028No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3150.12414478040167734No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3130.12335655957373018No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3130.12335655957373018No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGTCAATGGGTGAGAGCCTGAA3130.12335655957373018No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTAGTAGTC2980.11744490336412648No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC2930.11547435129425859No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC2910.11468613046631145No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT2910.11468613046631145No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGAGTAGTC2890.11389790963836428No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCTGTAGTC2880.1135037992243907No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTGGTAGTC2850.11232146798246997No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGGGTAGTC2800.11035091591260207No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC2790.1099568054986285No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT2770.10916858467068134No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGTGTAGTC2720.10719803260081345No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCGAGTAGTC2690.1060157013588927No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2680.10562159094491912No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2670.10522748053094556No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2670.10522748053094556No Hit
CCTACGGGGGGCAGCAGGATTAGATACCCCAGTAGTC2660.10483337011697197No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCGGTAGTC2630.10365103887505123No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2610.10286281804710408No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTTGTAGTC2580.10168048680518334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)