FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253736
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA118354.664296749377305No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG73802.908534855125012No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA70962.7966074975565154No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA67652.666156950531261No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA59162.3315572090676926No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA55742.1967714474887283No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG52032.0505564839045305No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA44191.7415739193492448No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA43051.6966453321562567No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA42421.6718163760759213No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG41231.6249172368130658No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG40411.592600182867232No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG40191.5839297537598136No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39571.5594949080934515No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG36691.4459911088690607No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG31331.2347479269792225No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG30261.1925781126840496No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG30151.1882428981303401No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27981.1027209382980736No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26431.041633824132169No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG26071.02744584922912No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG25461.0034051139767317No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24650.9714821704448718No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG24480.964782293407321No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG23430.9234006999400952No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG22440.8843837689567109No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21470.8461550588012737No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20130.7933442633288141No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG19070.7515685594476148No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17830.702698868114891No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG17540.6912696661096572No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG17400.6857521203140272No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG17370.6845697890721064No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG16490.6498880726424315No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14500.5714601002616894No Hit
GACTACTGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG14110.5560897941167198No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13900.5478134754232745No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13700.539931267143803No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG13440.5296843963804899No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA13430.5292902859665164No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13410.5285020651385692No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG11760.4634738468329287No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG9410.37085789954913767No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG8910.35115237885045875No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG8850.34878771636661726No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG8740.3444525018129079No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8340.32868808525396476No Hit
GACTACTCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG8320.3278998644260176No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG7930.312529558281048No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7760.30582968124349713No Hit
GACTACAGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG7710.3038591291736293No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG7640.3011003562758142No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7490.29518870006621056No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7480.294794589652237No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7400.29164170634044834No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG7240.2853359397168711No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7210.28415360847495036No Hit
GACTACTAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG7110.28021250433521455No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7100.27981839392124097No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7020.2766655106094524No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG6790.26760097108806No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6570.2589305419806413No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG6470.2549894378409055No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG6270.24710722956143394No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG6130.24158968376580384No Hit
GACTACTGGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA6050.23843680045401522No Hit
GACTACCGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5910.2329192546583851No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5900.2325251442444115No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5870.23134281300249077No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG5480.21597250685752117No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG5430.21400195478765333No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5320.20966674023394394No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG5290.2084844089920232No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG5170.20375508402434026No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG5170.20375508402434026No Hit
GACTACACGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5140.2025727527824195No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5070.19981397988460448No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG5060.1994198694706309No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG5000.1970552069867894No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4980.19626698615884228No Hit
GACTACCCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG4970.1958728757448687No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4880.19232588201910647No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG4830.1903553299492386No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG4810.18956710912129143No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4810.18956710912129143No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4800.18917299870731785No Hit
GACTACCAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG4780.1883847778793707No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG4700.18523189456758204No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4610.18168490084181985No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4600.18129079042784627No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4530.17853201753003123No Hit
GACTACAAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG4520.17813790711605765No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4500.1773496862881105No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG4470.17616735504618974No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG4450.17537913421824258No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTGCCTCAG4440.174985023804269No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4400.17340858214837468No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4400.17340858214837468No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4360.17183214049248038No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4320.17025569883658606No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4320.17025569883658606No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG4300.1694674780086389No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4250.167496925938771No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4210.1659204842828767No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3980.15685594476148437No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3870.152520730207775No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG3860.15212661979380143No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG3790.14936784689598637No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3770.1485796260680392No Hit
GACTACTGGGGTATCTAGATGAGGCAGGACTTCAAGGAAGGGGATGGATA3580.14109152820254123No Hit
GACTACTCGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA3500.1379386448907526No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3370.13281520950909606No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3370.13281520950909606No Hit
GACTACTAGGGTATCTAATCCTGCAGCCACCCGTAGG3260.1284799949553867No Hit
GACTACAGGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA3120.1229624491597566No Hit
GACTACTAGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA3100.12217422833180944No Hit
GACTACAGGGGTATCTAATCCTGCAGCCTCCCGTAGG3070.12099189708988871No Hit
GACTACTCGGGTATCTAATCCTGCAGCCGCCCGTAGG3040.11980956584796795No Hit
GACTACACGGGTATCTAATCCTGCAGCCCCCCGTAGG3000.11823312419207364No Hit
GACTACTGGGGTATCTAATCCTGCTGCCCCCCGTAGG3000.11823312419207364No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3000.11823312419207364No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2990.11783901377810006No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2960.11665668253617933No Hit
GACTACCAGGGTATCTAATCCTGCAGCCCCCCGTAGG2930.11547435129425859No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2920.11508024088028501No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2900.11429202005233786No Hit
GACTACCCGGGTATCTAATCCTGCAGCCCCCCGTAGG2900.11429202005233786No Hit
GACTACCGGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA2880.1135037992243907No Hit
GACTACCGGGGTATCTAATCCTGCAGCCACCCGTAGG2880.1135037992243907No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2850.11232146798246997No Hit
GACTACTCGGGTATCTAATCCTGCAGCCTCCCGTAGG2850.11232146798246997No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2790.1099568054986285No Hit
GACTACAAGGGTATCTAATCCTGCAGCCCCCCGTAGG2740.10798625342876059No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2720.10719803260081345No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2710.10680392218683987No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2710.10680392218683987No Hit
GACTACAGGGGTATCTAATCCTGCAGCCGCCCGTAGG2670.10522748053094556No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2640.10404514928902481No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2590.10207459721915692No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.10128637639120976No Hit
GACTACTAGGGTATCTAATCCTGCAGCCGCCCGTAGG2560.10089226597723618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)