FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194103
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA67453.4749591711617027No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG44292.28177823114532No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG42542.1916199131389003No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37581.936085480389278No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA36681.8897183454145479No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA34721.7887410292473582No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG32041.6506700051003849No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31241.609454774011736No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG27251.4038938089571No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA27201.4013178570140596No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG26131.3461924854329919No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24971.286430400354451No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG24451.2596405001468292No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG24241.248821501986059No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA23871.2297594576075588No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG23731.2225467921670454No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23471.2091518420632343No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22701.16948218214041No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22391.1535112800935585No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22341.150935328150518No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG21381.1014770508441396No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG21371.1009618604555313No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG19180.9881351653503552No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG18200.9376465072667605No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG18140.9345553649351117No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG17750.9144629397793955No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG17560.9046743223958413No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG17520.902613560841409No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG16660.8583071874211115No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG16580.8541856643122465No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG15640.8057577677830842No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG15600.8036970062286517No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG14610.7526931577564488No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG14470.7454804923159354No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14290.7362070653209893No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13950.7186905921083137No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13830.7125083074450163No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13790.7104475458905839No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13610.7011741188956379No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13550.6980829765639892No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13410.6908703111234757No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG13030.6712930763563675No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG12690.6537766031436918No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG12220.6295626548791106No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG11640.5996816123398402No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11540.5945297084537591No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG11510.5929841372879348No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11360.585256281458813No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11230.5785588064069077No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG10790.5558904293081509No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10630.547647383090421No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG10570.5445562407587724No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG10470.5394043368726913No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG10270.5291005291005291No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9990.514675198219502No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9910.5105536751106371No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG9690.4992194865612587No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG9130.47036882479920455No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8930.46006501702704233No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8680.44718525731183956No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8560.4410029726485422No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG8350.43018397448777196No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8270.426062451378907No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG8190.42194092827004215No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8020.4131826916637043No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG7890.4064852166117989No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7780.40081812233710973No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7760.3997877415598935No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7660.3946358376738124No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7650.39412064728520424No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG7530.38793836262190695No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7150.36836112785479874No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG7050.36320922396871763No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6870.35393579697377164No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG6750.34775351231047436No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG6480.33384337181805535No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5950.3065382812218255No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5920.3049927100560012No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5830.30035599655852824No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5810.299325615781312No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5660.29159775995219034No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5360.276142048293947No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5080.26171671741291996No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4870.25089771925214965No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4860.2503825288635415No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4690.24162429225720364No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4680.24110910186859552No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4590.23647238837112253No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4440.2287445325420009No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4360.224623009433136No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTACCTCAG4250.21895591515844678No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4120.21225844010654138No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3830.1973179188369062No Hit
GACTACTGGGGTATCTAATCCACATAAACACCCAGGATTGCTGGGATAGC3780.19474196689386564No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3520.18134701679005477No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3510.18083182640144665No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3370.1736191609609331No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3370.1736191609609331No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3180.16383054357737903No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3040.15661787813686548No Hit
GACTACTGGGGTATCTAATCCCATCTAGCTCTCAGTGCCCTGACCTCTGT2870.1478596415305276No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2710.13961659531279785No Hit
GACTACTGGGGTATCTATAGATTCCAGACATTGTATGGAAAAGCACTGTG2640.13601026259254106No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2640.13601026259254106No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTACCTCAG2630.13549507220393295No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTACCTCAG2570.1324039298722843No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2500.12879759715202752No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG2230.11488745665960856No Hit
GACTACTGGGGTATCTAATCCATTCATCACACTTCCAGCAAAGCAGTCAT2200.11334188549378423No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTACCTCAG2190.11282669510517612No Hit
GACTACTGGGGTATCTAATCCACATGGTAGCAAGATGGCAGTCTGTATCT2170.11179631432795989No Hit
GACTACTGGGGTATCTGGACTCAGATATGGAATAAAGTGTGTGTAGCCTG2080.10715960083048691No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTACCTCAG2060.10612922005327069No Hit
GACTACTCGGGTATCTAATCCACATAAACACCCAGGATTGCTGGGATAGC2010.10355326811023013No Hit
GACTACAGGGGTATCTAATCCACATAAACACCCAGGATTGCTGGGATAGC2010.10355326811023013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)