FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276028
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA163205.912443665135421No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA93843.3996551074528667No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA92223.340965409306302No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA80152.9036909299056615No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG72642.6316170823249814No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA66192.3979451360006956No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA57902.0976132856087064No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56822.0584868201776634No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA55292.0030576608170185No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA53451.9363977567493154No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG46781.694755604503891No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG42471.5386120248670425No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG41591.5067312011824887No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG37401.354935006593534No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG36531.3234164649963047No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG30461.103511237990349No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG27500.9962757401423044No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25980.9412088628689842No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG25410.9205587838914892No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG24900.9020823974379412No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG23360.846290955989972No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG23060.8354224933702378No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG22420.8122364397814715No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21450.7770950773109974No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21430.7763705131363485No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20730.7510107670236352No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19140.6934079151390439No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18340.664425348153086No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16300.5905198023388932No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16300.5905198023388932No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16240.5883461098149463No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16060.5818250322431058No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14930.5408871563754402No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14470.5242221803585144No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG13580.4919790745866361No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12920.46806845682322085No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12510.45321489124291736No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11970.4336516585273958No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG10670.38655498717521414No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG9970.3611952410625009No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9290.33656005912443665No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG9160.3318503919892185No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7930.2872896952483081No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG7890.2858405668990103No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG7230.2619299491355949No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG7150.25903169243699914No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG6870.24888779399191385No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6780.24562725520599357No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG6680.24200443433274885No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG6300.22823771501441883No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6260.22678858666512094No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG6140.22244120161722725No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6010.21773153448200908No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG5750.20831220021157276No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG5730.2075876360369238No Hit
GACTACTGGGGTATCTAATCCTTGCAGCCCCCCGTAGG5640.20432709725100354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG5550.20106655846508326No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5480.19853058385381192No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG5310.1923717883692959No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG5270.19092266001999797No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5270.19092266001999797No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5210.18874896749605113No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG5180.18766212123407772No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5060.183314736186184No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG4900.17751822278899243No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4860.17606909443969454No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4860.17606909443969454No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4670.1691857347805295No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4630.16773660643123162No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4530.16411378555798686No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG4380.15867955424811975No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG4260.15433216920022608No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4250.15396988711290158No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG4240.1536076050255771No Hit
GACTACTGGGGTATCTAATCCTTGCAGCCACCCGTAGG4190.15179619458895474No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4170.1510716304143058No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG4070.14744880954106104No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3980.1441882707551408No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3900.14129001405654498No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG3880.14056544988189604No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG3800.13766719318330023No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3780.13694262900865128No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG3730.13513121857202892No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3730.13513121857202892No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3730.13513121857202892No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3710.13440665439737998No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG3700.1340443723100555No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3680.13331980813540656No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3660.1325952439607576No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3660.1325952439607576No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3660.1325952439607576No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3500.12679873056356603No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3460.1253496022142681No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG3450.12498732012694365No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3450.12498732012694365No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG3420.1239004738649702No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3420.1239004738649702No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGTCTCAG3400.12317590969032127No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG3360.12172678134102337No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3340.12100221716637442No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG3270.1184662425551031No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG3260.11810396046777864No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG3250.11774167838045416No Hit
GACTACTCGGGTATCTAATCCTTGCAGCCCCCCGTAGG3250.11774167838045416No Hit
GACTACTGGGGTATCTAATCCTTGCAGCCGCCCGTAGG3250.11774167838045416No Hit
GACTACAGGGGTATCTAATCCTTGCAGCCCCCCGTAGG3160.11448113959453389No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3130.11339429333256047No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3100.11230744707058705No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3060.11085831872128915No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3050.11049603663396466No Hit
GACTACTAGGGTATCTAATCCTTGCAGCCCCCCGTAGG3050.11049603663396466No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3010.10904690828466679No Hit
GACTACTGGGGTATCTAATCCTCTGCTCATTTTGCTGCAATTCGATATCA3000.1086846261973423No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2980.10796006202269334No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2980.10796006202269334No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2970.10759777993536888No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2950.10687321576071993No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG2910.10542408741142203No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG2900.10506180532409756No Hit
GACTACTGGGGTATCTAATCCCACATAACAAAGATGCCATGCAAGCAGGCCTGCAGCCCCCCGTAGG2900.10506180532409756No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG2890.10469952323677308No Hit
GACTACTGGGGTATCTAATCCTTGCAGCCTCCCGTAGG2880.10433724114944862No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2860.10361267697479966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)