FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286505
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC203217.092720894923301No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC187896.558000732971501No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT154725.400254794855238No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT147685.154534824872167No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC143655.013874103418789No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC126264.406903893474809No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT103713.619832114622781No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT102803.5880700162300836No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC66152.3088602293153695No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT49151.7155023472539745No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC42331.4774611263328736No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT33141.156698835971449No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC32451.1326154866407219No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28400.9912566970908011No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC24500.8551334182649518No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23210.8101080260379401No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20390.7116804244254027No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20170.7040016753634317No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19130.6677021343432052No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC17430.6083663461370656No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14760.5151742552485995No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14450.5043541997521858No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13540.4725921013594876No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10550.36823092092633636No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT9920.34624177588523763No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9900.34554370778869475No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT9620.33577075443709536No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9380.3273939372785815No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8730.30470672414093997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8410.29353763459625487No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCCAGTAGTC6970.243276731645172No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT6810.23769218687282942No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT6230.21744821207308773No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT6210.2167501439765449No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6150.21465593968691643No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5900.20593008848013125No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT5850.20418491823877416No Hit
CCTACGGGTGGCTGCAGAGGATTAGATACCCCAGTAGTC5470.19092162440446067No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5470.19092162440446067No Hit
CCTACGGGGGGCTGCAGAAGCGAGGATGTGGATTAGATACCCCAGTAGTC5060.1766112284253329No Hit
CCTACGGGGGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC4930.17207378579780458No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4810.16788537721854768No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4620.1612537303013909No Hit
CCTACGGGTGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC4460.15566918552904838No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4320.15078270885324863No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4220.14729236837053455No Hit
CCTACGGGTGGCTGCAGAAGCGAGGATGTGGATTAGATACCCCAGTAGTC4070.14205685764646342No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCCTGTAGTC4070.14205685764646342No Hit
CCTACGGGCGGCTGCAGAGGATTAGATACCCCAGTAGTC3970.13856651716374932No Hit
CCTACGGGAGGCTGCAGAGGATTAGATACCCCAGTAGTC3960.13821748311547793No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3830.1336800404879496No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT3820.13333100643967818No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT3720.1298406659569641No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCTAGTAGTC3700.1291425978604213No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3660.12774646166733566No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCGAGTAGTC3510.12251095094326452No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3480.12146384879845029No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3470.12111481475017888No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3410.11902061046055043No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3400.118671576412279No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3350.11692640617092197No Hit
CCTACGGGAGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC3330.11622833807437916No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3290.11483220188129352No Hit
CCTACGGGGGGCTGCAGAAGCGAGGATGTGGATTAGATACCCGAGTAGTC3270.11413413378475071No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC3250.1134360656882079No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3230.11273799759166506No Hit
CCTACGGGAGGCTGCAGAAGCGAGGATGTGGATTAGATACCCCAGTAGTC3200.11169089544685083No Hit
CCTACGGGCGGCTGCAGAAGCGAGGATGTGGATTAGATACCCCAGTAGTC3180.11099282735030802No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3150.1099457252054938No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT3130.10924765710895099No Hit
CCTACGGGGTGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3060.10680441877105112No Hit
CCTACGGGCGGCTGCAGCTCTCCACCAAAGGTCACAAAGGTAAGTACCTC3000.10471021448142266No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3000.10471021448142266No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2970.10366311233660844No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCCGGTAGTC2900.10121987399870856No Hit
CCTACGGGGGGCTGCGAGAGGATTAGATACCCCAGTAGTC2880.10052180590216575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)