FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286505
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA201717.040365787682588No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA113963.977592014100976No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA113023.9447828135634633No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA102403.5741086542992266No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG99533.4739358824453324No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA84552.951082878134762No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA73072.5503917907191846No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA68342.3852986858868084No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA67592.3591211322664525No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA65762.295247901432785No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG57181.995776688015916No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG56131.959128112947418No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG49911.742028934922602No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG44391.549362140276784No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG36181.2628051866459573No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG34601.2076578070190747No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG33811.1800841172056333No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG31661.1050417968272805No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG26650.9301757386433046No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20660.7211043437287308No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG16250.5671803284410394No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG15970.55740737508944No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG15770.5504266941240118No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG14300.49911868902811474No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13220.46142301181480255No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12890.449904888221846No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12720.4439713094012321No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG11260.3930123383536064No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11060.38603165738817824No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10480.36578768258843647No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG9890.34519467374042334No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG9760.3406572311128951No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9600.3350726863405525No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG9160.31971518821661055No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8970.31308354129945376No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG8840.30854609867192545No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8720.30435769009266855No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG8710.30400865604439714No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8440.2945847367410691No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8280.28900019196872656No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8230.2872550217273695No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8230.2872550217273695No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7960.27783110242404147No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7770.2711994555068847No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG7610.2656149107345422No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7470.2607284340587424No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7410.258634229769114No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7210.25165354880368584No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6650.2321076421004869No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6640.2317586080522155No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG6030.21046753110765953No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5950.2076752587214883No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5890.20558105443185984No Hit
GACTACTGGGGTATCTAATCCACATCCTCGCTTCTGCAGCCCCCCGTAGG5810.20278878204568856No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG5800.20243974799741712No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG5700.19894940751470305No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5640.1968552032250746No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCACCCGTAGG5530.19301582869408912No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5510.1923177605975463No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG5290.1846390115355753No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5190.1811486710528612No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5130.17905446676323275No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4980.17381895603916162No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4750.1657911729289192No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4700.16404600268756217No Hit
GACTACTGGGGTATCTAGATGAGGCAGGACTTCAAGGAAGGGGATGGATA4610.1609046962531195No Hit
GACTACTGGGGTATCTAATCCACATCCTCGCTTCTGCAGCCACCCGTAGG4480.15636725362559117No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4390.1532259471911485No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4350.15182981099806286No Hit
GACTACAGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG4300.1500846407567058No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4230.14764140241880597No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4210.14694333432226314No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4200.14659430027399173No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4130.14415106193609187No Hit
GACTACTAGGGTATCTAATCCTCTGCAGCCCCCCGTAGG4060.141707823598192No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCTCCCGTAGG4020.14031168740510638No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4020.14031168740510638No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCGCCCGTAGG3940.13751941501893508No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3890.13577424477757805No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3850.13437810858449242No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3850.13437810858449242No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3800.1326329383431354No Hit
GACTACTCGGGTATCTAATCCTCTGCAGCCCCCCGTAGG3740.1305387340535069No Hit
GACTACTCGGGTATCTAATCCACATCCTCGCTTCTGCAGCCCCCCGTAGG3650.12739742761906425No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3620.12635032547425No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3610.1260012914259786No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG3560.12425612118462157No Hit
GACTACTGGGGTATCTAATCCACATCCTCGCTTCTGCAGCCGCCCGTAGG3540.12355805308807875No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3410.11902061046055043No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3400.118671576412279No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3370.11762447426746478No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3360.11727544021919338No Hit
GACTACCGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG3330.11622833807437916No Hit
GACTACTGGGGTATCTAATCCACATCCTCGCTTCTGCAGCCTCCCGTAGG3310.11553026997783633No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG3300.11518123592956493No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3210.11203992949512226No Hit
GACTACTGGGGTATCTAATCCTCTCGCAGCCCCCCGTAGG3170.11064379330203661No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG3170.11064379330203661No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3160.11029475925376521No Hit
GACTACTAGGGTATCTAATCCACATCCTCGCTTCTGCAGCCCCCCGTAGG3110.10854958901240816No Hit
GACTACAGGGGTATCTAATCCACATCCTCGCTTCTGCAGCCCCCCGTAGG3050.1064553847227797No Hit
GACTACCAGGGTATCTAATCCTCTGCAGCCCCCCGTAGG2870.10017277185389434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)