FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212803
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT59532.7974229686611563No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC57542.7039092493996795No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT53662.5215809927491626No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC53592.518291565438457No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC39021.8336207666245306No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38511.809654939075107No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT38361.802606166266453No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC38141.7922679661470937No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT37571.7654826294742088No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC35281.657871364595424No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC32321.518775581171318No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT27041.2706587783066967No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC23211.0906801125923977No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC23191.0897402762179105No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT23001.080811830660282No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC19660.9238591561209193No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19140.8994234103842522No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC17720.8326950277956607No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17690.83128527323393No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17420.8185974821783527No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT16450.7730154180157235No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT16330.7673763997688002No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT15220.7152154809847605No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT14410.6771521078180288No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT13340.6268708617829636No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC13100.6155928252891172No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13060.6137131525401428No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12870.6047847069825143No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT12680.5958562614248859No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12440.5845782249310395No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC12360.5808188794330907No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT11880.5582628064453978No Hit
CCTACGGGGGGCTGCAGTTAGGAATCTTCCGCAATGGGCGAAAGCCTGAC11520.5413457517046283No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT11450.538056324393923No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11300.531007551585269No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT10120.47555720549052416No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9620.452061296128344No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT9300.43702391413654884No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT9290.4365539959493053No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9240.4342044050130872No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT9230.43373448682584365No Hit
CCTACGGGTGGCTGCAGTTAGGAATCTTCCGCAATGGGCGAAAGCCTGAC9170.430914977702382No Hit
CCTACGGGGGGCTGCAGTTGGGAATTTTGGACAATGGGGGAAACCCTGAT9080.42668571401718963No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT9030.42433612308097163No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT8900.4182271866468048No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT8580.40318980465500953No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT8570.40271988646776596No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT8570.40271988646776596No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8400.39473127728462476No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT8220.38627274991423993No Hit
CCTACGGGAGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT8000.3759345497948807No Hit
CCTACGGGTGGCTGCAGTTGGGAATTTTGGACAATGGGGGAAACCCTGAT7980.37499471342039353No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGACGCAAGTCTGAC7620.3580776586796239No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7530.3538483949944315No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT7490.35196872224545706No Hit
CCTACGGGCGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT7480.35149880405821343No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7300.34304027668782866No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT7220.33928093118987984No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT7080.33270207656846945No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6940.326123221947059No Hit
CCTACGGGAGGCTGCAGTTAGGAATCTTCCGCAATGGGCGAAAGCCTGAC6910.32471346738532825No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6910.32471346738532825No Hit
CCTACGGGGGGCTGCAGTCAAGAAACTTTGACAATGCACGAAAGTGTGAT6870.3228337946363538No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT6830.3209541218873794No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT6820.3204842037001358No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT6570.3087362490190458No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCGCAATGGACGCAAGTCTGAC6450.3030972307721226No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6350.2983980488996866No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT6280.2951086215889814No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6200.29134927609103256No Hit
CCTACGGGCGGCTGCAGTTAGGAATCTTCCGCAATGGGCGAAAGCCTGAC6150.28899968515481456No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5810.2730224667885321No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5780.2716127122268013No Hit
CCTACGGGAGGCTGCAGTTGGGAATTTTGGACAATGGGGGAAACCCTGAT5770.2711427940395577No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT5730.26926312129058333No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT5610.2636241030436601No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC5550.2608045939201985No Hit
CCTACGGGTGGCTGCAGTCAAGAAACTTTGACAATGCACGAAAGTGTGAT5480.2575151666094933No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT5430.25516557567327525No Hit
CCTACGGGCGGCTGCAGTTGGGAATTTTGGACAATGGGGGAAACCCTGAT5370.2523460665498137No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCGCAATGGACGCAAGTCTGAC5010.23542901180904405No Hit
CCTACGGGCGGCTGCAGTCAAGAAACTTTGACAATGCACGAAAGTGTGAT4740.2227412207534668No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT4680.21992171163000523No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4670.2194517934427616No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT4640.2180420388810308No Hit
CCTACGGGGTGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT4590.2156924479448128No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4530.2128729388213512No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT4420.2077038387616716No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4420.2077038387616716No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA4390.20629408419994077No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4340.2039444932637228No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCGCAATGGACGCAAGTCTGAC4330.2034745750764792No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4310.20253473870199198No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4310.20253473870199198No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT4080.19172662039538918No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4000.18796727489744036No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA3890.18279817483776076No Hit
CCTACGGGAGGCTGCAGTCAAGAAACTTTGACAATGCACGAAAGTGTGAT3850.18091850208878632No Hit
CCTACGGGGGGCTGCAGTCGAGAATATTCGACAATGGACGAAAGTCTGAT3810.17903882933981194No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3750.17621932021635034No Hit
CCTACGGGGGGCAGCAGTTAGGAATCTTCCGCAATGGGCGAAAGCCTGAC3650.17152013834391433No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3480.1635315291607731No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC3460.1625916927862859No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3450.16212177459904228No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3380.1588323472883371No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTGAGCCTGAA3330.1564827563521191No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT3320.1560128381648755No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT3300.1550730017903883No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3300.1550730017903883No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT3220.15131365629243948No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT3150.14802422898173428No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3120.14661447442000347No Hit
CCTACGGGGTGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3010.14144537436032387No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2970.13956570161134946No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA2930.13768602886237508No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT2850.13392668336442626No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2830.13298684698993907No Hit
CCTACGGGGGGCAGCAGTTGGGAATTTTGGACAATGGGGGAAACCCTGAT2810.13204701061545185No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2790.13110717424096466No Hit
CCTACGGGTGGCTGCAGTCGAGAATATTCGACAATGGACGAAAGTCTGAT2760.12969741967923384No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2760.12969741967923384No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2660.12499823780679784No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2660.12499823780679784No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTGAGCCTGAA2650.12452831961955423No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA2640.12405840143231064No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC2610.12264864687057983No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2580.12123889230884902No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT2580.12123889230884902No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT2570.12076897412160544No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2540.11935921955987462No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2520.11841938318538743No Hit
CCTACGGGTGGCTGCAGTCGAGAATATTCCACAATGGACGAAAGTCTGAT2390.11231044675122061No Hit
CCTACGGGTGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2380.11184052856397701No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAC2340.10996085581500262No Hit
CCTACGGGCGGCTGCAGTCGAGAATATTCGACAATGGACGAAAGTCTGAT2330.10949093762775901No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2320.1090210194405154No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2190.10291208300634859No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTGAGCCTGAA2180.10244216481910498No Hit
CCTACGGGAGGCTGCAGTCGAGAATATTCGACAATGGACGAAAGTCTGAT2170.1019722466318614No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2160.10150232844461779No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2160.10150232844461779No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2140.10056249207013059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)