FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272734
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC93283.4201823021698794No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC77212.830963502900262No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC62692.2985766351096673No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT57032.09104842080562No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC54952.014783635336995No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT54221.988017628898487No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA45141.6550925077181429No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT41011.5036629096482286No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT38841.4240982055775957No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA38691.418598341240916No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC34011.2470025739365096No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT28931.0607405017342906No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT28391.0409409901222437No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA28381.0405743324997985No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28191.0336078376733373No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA28051.028474630959103No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT25580.9379101982151108No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC24250.8891447344298842No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT24250.8891447344298842No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC21590.7916138068594308No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT20680.7582479632169072No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC20150.7388151092273058No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT18390.6742833676769306No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT18280.6702501338300322No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT17890.655950486554665No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC17750.6508172798404306No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC17140.6284511648712665No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT17070.6258845615141493No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA17000.6233179581570322No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT16980.6225846429121414No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16940.6211180124223602No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT14880.5455865421986258No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14870.5452198845761804No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT14410.528353633943696No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC14250.5224871119845711No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT13540.4964544207909538No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13480.49425447505628195No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC13200.4839880616278132No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC13090.4799548277809147No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC12920.4737216481993444No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT12570.46088863141375846No Hit
CCTACGGGGGGCTGCAGTCGAGAATATTTCGCAATGGGGGAAACCCTGAC12220.44805561462817245No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT11970.4388891740670397No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA11260.41285648287342247No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10900.3996568084653912No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10740.39379028650626624No Hit
CCTACGGGTGGCTGCAGTCGAGAATATTTCGCAATGGGGGAAACCCTGAC10170.37289080202688335No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT9510.34869139894549267No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9380.3439248498537036No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9340.34245821936392234No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9310.3413582464965864No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9150.3354917245374614No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA8630.31642552817030517No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT8580.31459224005807856No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8430.30909237572139886No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8350.3061591147418364No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8330.30542579949694576No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8250.3024925385173832No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC8100.2969926741807035No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCTCAATGGGCGCAAGCCTGAA7980.29259278271135974No Hit
CCTACGGGGGGCTCAGGATTAGATACCCCAGTAGTC7950.2914928098440238No Hit
CCTACGGGCGGCTGCAGTCGAGAATATTTCGCAATGGGGGAAACCCTGAC7920.2903928369766879No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT7830.2870929183746801No Hit
CCTACGGGAGGCTGCAGTCGAGAATATTTCGCAATGGGGGAAACCCTGAC7700.282326369282891No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA7490.27462655921153944No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7470.2738932439666488No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCTCAATGGGCGCAAGCCTGAA7460.2735265863442035No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7430.2724266134768676No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT7080.2595935966912816No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7050.2584936238239457No Hit
CCTACGGGGGGCTGCAGTCGAGAATATTCCACAATGGGCGAAAGCCTGAT6870.25189378661993006No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC6310.2313609597629925No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6100.22366114969164094No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6030.22109454633452374No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5980.21926125822229717No Hit
CCTACGGGTGGCTCAGGATTAGATACCCCAGTAGTC5910.21669465486518No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5870.21522802437539873No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5850.2144947091305081No Hit
CCTACGGGTGGCTGCAGTCGAGAATATTCCACAATGGGCGAAAGCCTGAT5780.21192810577339094No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5450.1998284042326956No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5220.1913952789164534No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCTCAATGGGCGCAAGCCTGAA5140.18846201793689088No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5130.18809536031444557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5080.186262072202219No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5050.18516209933488306No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT5030.18442878408999247No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5020.18406212646754713No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5000.1833288112226565No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5000.1833288112226565No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCTCAATGGGCGCAAGCCTGAA4950.18149552311042993No Hit
CCTACGGGGGGCTCAGGATTAGATACCCGAGTAGTC4900.17966223499820336No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT4770.1748956859064143No Hit
CCTACGGGCGGCTGCAGTCGAGAATATTCCACAATGGGCGAAAGCCTGAT4680.17159576730440648No Hit
CCTACGGGAGGCTGCAGTCGAGAATATTCCACAATGGGCGAAAGCCTGAT4670.17122910968196117No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4670.17122910968196117No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4570.16756253345750804No Hit
CCTACGGGGGGCTCAGGATTAGATACCCCTGTAGTC4570.16756253345750804No Hit
CCTACGGGGGGCTCAGGATTAGATACCCTAGTAGTC4530.1660959029677268No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4530.1660959029677268No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4470.16389595723305492No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4260.15619614716170332No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4250.15582948953925804No Hit
CCTACGGGTGGCTGCAGCCGAGAATATTCCGCAATGGGCGCAAGCCTGAC4170.1528962285596955No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4050.14849633709035176No Hit
CCTACGGGGGGCTGCAGCCGAGAATATTCCGCAATGGGCGCAAGCCTGAC4040.14812967946790645No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3970.14556307611078928No Hit
CCTACGGGGGGCTCAGGATTAGATACCCCGGTAGTC3860.14152984226389081No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC3820.14006321177410958No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3790.13896323890677365No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3610.132363401702758No Hit
CCTACGGGCGGCTCAGGATTAGATACCCCAGTAGTC3590.13163008645786736No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3510.12869682547830488No Hit
CCTACGGGAGGCTCAGGATTAGATACCCCAGTAGTC3470.12723019498852361No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3440.12613022212118766No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA3410.12503024925385173No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCTCAATGGGCGCAAGCCTGAA3390.12429693400896112No Hit
CCTACGGGTGGCTCAGGATTAGATACCCCTGTAGTC3330.12209698827428923No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3330.12209698827428923No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3330.12209698827428923No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3260.11953038491717204No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3240.11879706967228142No Hit
CCTACGGGGGGCTGCAGAAGCTTGGATCGGATTAGATACCCCAGTAGTC3220.11806375442739078No Hit
CCTACGGGGGGCTCAGGATTAGATACCCTGGTAGTC3180.11659712393760954No Hit
CCTACGGGGGGCAGCAGTCGAGAATATTTCGCAATGGGGGAAACCCTGAC3170.11623046631516422No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT3170.11623046631516422No Hit
CCTACGGGCGGCTGCAGCCGAGAATATTCCGCAATGGGCGCAAGCCTGAC3140.11513049344782829No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT3120.11439717820293765No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3100.11366386295804703No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3090.11329720533560173No Hit
CCTACGGGCGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC3030.11109725960092984No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT2990.10963062911114858No Hit
CCTACGGGAGGCTGCAGCCGAGAATATTCCGCAATGGGCGCAAGCCTGAC2940.10779734099892202No Hit
CCTACGGGTGGCTCAGGATTAGATACCCGAGTAGTC2910.10669736813158609No Hit
CCTACGGGAGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC2870.10523073764180484No Hit
CCTACGGGTGGCTCAGGATTAGATACCCCGGTAGTC2850.1044974223969142No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT2850.1044974223969142No Hit
CCTACGGGGGGCTCAGGATTAGATACCCGGGTAGTC2830.10376410715202358No Hit
CCTACGGGTGGCTCAGGATTAGATACCCTAGTAGTC2820.10339744952957827No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2820.10339744952957827No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2810.10303079190713295No Hit
CCTACGGGGGGCTCAGGATTAGATACCCGTGTAGTC2760.10119750379490638No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGACAATGGGGGCAACCCTGAT2750.10083084617246109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)