FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272734
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA98433.6090109777292163No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG60582.221211876773706No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG57092.093248366540292No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56362.066482360101784No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56312.0646490719895576No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA52511.9253191754603387No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG45481.6675588668812837No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA41551.5234624212602754No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA36091.3232673594051347No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG34751.2741352379974626No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG34731.2734019227525721No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG33661.2341695571509235No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32961.2085035235797519No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32591.194937191549275No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32541.1931039034370485No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31871.1685378427332127No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG30871.1318720804886813No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29941.0977729216012673No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28011.0270080004693216No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27020.9907088958472359No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG26690.9786091943065404No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG25710.9426767473068998No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG24960.9151774256235012No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24220.8880447615625481No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG23800.872645141419845No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG20870.7652144580433683No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20460.7501814955231104No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG19950.7314819567783994No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19480.7142490485234697No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19380.7105824722990166No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG19020.6973827978909853No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG18990.6962828250236494No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG18700.6856497539727353No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18680.6849164387278447No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG16360.5998518703205321No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG15660.5741858367493602No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG15330.5620861352086648No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG15090.5532863522699774No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG15070.5525530370250867No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG14940.5477864879332976No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14490.5312868949232585No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12480.4575887128117506No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12400.45465545183218814No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG12300.45098887560773504No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA11620.42605615728145374No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11140.4084565914040787No Hit
GACTACTGGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG10150.37215748678199273No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9480.34759142607815674No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG9350.34282487698636765No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9310.3413582464965864No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG9170.336225039782352No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9070.3325584635578989No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8880.3255919687314379No Hit
GACTACTGGGGTATCTAATCCTGAGCCCCCCGTAGG8770.3215587348845395No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8580.31459224005807856No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG7560.2771931625686566No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7540.276459847323766No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7250.26582677627285195No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7240.2654601186504066No Hit
GACTACTGGGGTATCTAATCCTTTTCTCTCCCCACGCTTTCGCGGCTCAG7240.2654601186504066No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG7240.2654601186504066No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7240.2654601186504066No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG7240.2654601186504066No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6930.2540937323546019No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6910.2533604171097113No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG6680.24492729179346912No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6600.2419940308139066No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6500.23832745458945348No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA6270.22989432927321127No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA6240.2287943564058753No Hit
GACTACTGGGGTATCTAATCCTGAGCCACCCGTAGG6200.22732772591609407No Hit
GACTACAGGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG5900.21632799724273466No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA5680.2082615295489378No Hit
GACTACTCGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG5630.20642824143671124No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG5610.20569492619182062No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5470.2005617194775862No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG5450.1998284042326956No Hit
GACTACTCGGGTATCTAATCCTGAGCCCCCCGTAGG5390.1976284584980237No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG5260.19286190940623463No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG5190.19029530604911746No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5150.18882867555933622No Hit
GACTACAGGGGTATCTAATCCTGAGCCCCCCGTAGG5060.1855287569573284No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5020.18406212646754713No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG4970.18222883835532058No Hit
GACTACTAGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG4970.18222883835532058No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4940.18112886548798465No Hit
GACTACTAGGGTATCTAATCCTGAGCCCCCCGTAGG4890.17929557737575808No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4860.17819560450842212No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG4760.174529028283969No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4720.17306239779418775No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4600.16866250632484397No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4580.16792919107995335No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG4560.16719587583506273No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG4550.16682921821261743No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTACCCACGCTTTCGAGCCTCAG4530.1660959029677268No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA4520.1657292453452815No Hit
GACTACAGGGGTATCTAATCCTTTTCTCTCCCCACGCTTTCGCGGCTCAG4500.16499593010039085No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG4480.16426261485550023No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG4430.16242932674327368No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4410.16169601149838303No Hit
GACTACTCGGGTATCTAATCCTTTTCTCTCCCCACGCTTTCGCGGCTCAG4380.1605960386310471No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4340.15912940814126586No Hit
GACTACCGGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG4340.15912940814126586No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4270.15656280478414866No Hit
GACTACCGGGGTATCTAATCCTGAGCCCCCCGTAGG4240.15546283191681273No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4240.15546283191681273No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4230.1550961742943674No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4170.1528962285596955No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4150.15216291331480492No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG4120.15106294044746899No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4070.1492296523352424No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4010.14702970660057052No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG3980.1459297337332346No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA3970.14556307611078928No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG3970.14556307611078928No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA3950.14482976086589863No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA3950.14482976086589863No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3910.1433631303761174No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCAG3810.13969655415166427No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG3740.13712995079454707No Hit
GACTACTAGGGTATCTAATCCTTTTCTCTCCCCACGCTTTCGCGGCTCAG3740.13712995079454707No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3720.13639663554965645No Hit
GACTACTGGGGTATCTAATCCTGAGCCGCCCGTAGG3700.13566332030476583No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG3690.1352966626823205No Hit
GACTACTGGGGTATCTAATCCTGAGCCTCCCGTAGG3670.13456334743742987No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG3610.132363401702758No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3550.13016345596808612No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG3520.1290634831007502No Hit
GACTACAGGGGTATCTAATCCTGAGCCACCCGTAGG3510.12869682547830488No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3490.12796351023341423No Hit
GACTACTGGGGTATCTAATCCGATCCAAGCTTCTGCAGCCCCCCGTAGG3470.12723019498852361No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG3460.1268635373660783No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTTCTTTCA3450.126496879743633No Hit
GACTACCAGGGTATCTAATCCTGAGCCCCCCGTAGG3410.12503024925385173No Hit
GACTACCAGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG3350.12283030351917985No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG3340.12246364589673456No Hit
GACTACACGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG3260.11953038491717204No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3240.11879706967228142No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3190.11696378156005485No Hit
GACTACCCGGGTATCTAATCCTGAGCCCCCCGTAGG3130.11476383582538296No Hit
GACTACACGGGTATCTAATCCTGAGCCCCCCGTAGG3120.11439717820293765No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3070.1125638900907111No Hit
GACTACTCGGGTATCTAATCCTGAGCCACCCGTAGG3060.11219723246826578No Hit
GACTACAAGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG3040.11146391722337515No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3030.11109725960092984No Hit
GACTACCCGGGTATCTAATCCCATTCGATTCCCACGCTTTCGTGTTTCAG3020.11073060197848454No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG3000.10999728673359391No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2990.10963062911114858No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG2980.10926397148870329No Hit
GACTACCGGGGTATCTAATCCTGAGCCACCCGTAGG2970.10889731386625798No Hit
GACTACAAGGGTATCTAATCCTGAGCCCCCCGTAGG2960.10853065624381265No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTCTCAG2940.10779734099892202No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2930.10743068337647672No Hit
GACTACTAGGGTATCTAATCCTGAGCCACCCGTAGG2920.1070640257540314No Hit
GACTACCGGGGTATCTAATCCTTTTCTCTCCCCACGCTTTCGCGGCTCAG2860.10486408001935951No Hit
GACTACTGGGGTATCTAATCCGCCGTTCTCCAGCCTGCAGCCCCCCGTAGG2860.10486408001935951No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2840.1041307647744689No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTACCCACGCTTTCGAGCCTCAG2780.10193081903979702No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2740.10046418855001576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)