FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264371
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT137325.1942157044456465No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT130624.940783974036487No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT96843.6630341451974684No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT91463.459532248242054No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC70092.6511985051310467No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC62002.3451891470698376No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC51921.963906782513967No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC48231.8243302026319075No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC42521.6083458473130565No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT41831.5822461616440533No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC39991.5126469998600451No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT35621.3473489906230258No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT31711.1994507718320089No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC30171.1411992994693063No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC29311.1086692564615634No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT27861.053822090925253No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC27741.0492830151567305No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT25900.9796838533727225No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC25040.9471538103649795No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23250.8794459301511891No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23150.8756633670107539No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22690.8582635765647517No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT22000.8321638908957488No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21940.8298943530114877No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC19320.7307911987320849No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC19230.7273868919056932No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC18230.6895612605013409No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17970.6797265963362094No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17140.648331322270597No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16870.638118401791422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC15720.5946189256764168No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT15580.5893233372798076No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15040.5688974963214574No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14590.5518759621894989No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13250.5011896161076669No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC13130.49665054033914463No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT13080.494759258768927No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC12220.46222921576118414No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT11900.45012501371179137No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC11900.45012501371179137No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT11290.42705137855513653No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC10910.4126776386214827No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10280.3888474908367408No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10030.3793910829856527No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9720.36766513725030353No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT9430.3566957041430414No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9330.3529131410026062No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT8570.3241656611352985No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT7720.2920138744415991No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7660.28974433655733794No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7520.2844487481607287No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7390.2795314160781629No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7370.27877490345007583No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7260.2746140839955971No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7120.2693184955989878No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC7120.2693184955989878No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC6990.264401163516422No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6820.2579708061776821No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6560.24813614201255058No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGTCAATGGACGCAAGTCTGAA6520.24662311675637646No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT6390.24170578467381065No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6380.24132752835976712No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC6290.23792322153337545No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6130.2318711205086791No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC6070.22960158262441796No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6030.22808855736824388No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC6020.22771030105420034No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5970.22581901948398272No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5850.22127994371546048No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5690.21522784269076412No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5460.20652794746776312No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5270.1993410775009362No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC5240.1982063085588056No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5160.19518025804645744No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5160.19518025804645744No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGTCAATGGACGCAAGTCTGAA5130.19404548910432687No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC5060.1913976949060222No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC4940.18685861913749996No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4910.18572385019536938No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC4860.18383256862515177No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4830.18269779968302122No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4600.1739979044600202No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4400.16643277817914975No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4400.16643277817914975No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4370.16529800923701918No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4370.16529800923701918No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGTCAATGGACGCAAGTCTGAA4320.1634067276668016No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC4140.15659811401401819No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4100.1550850887578441No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4050.15319380718762649No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4040.15281555087358295No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC3970.1501677566752783No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3930.1486547314191042No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGTCAATGGACGCAAGTCTGAA3900.14751996247697366No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3840.1452504245927125No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3830.14487216827866897No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3810.14411565565058193No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC3760.14222437408036434No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGACGCAAGTCTGAC3760.14222437408036434No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3690.13957657988205968No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3410.12898540308884104No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCGCAATGGCCGAAAGGCTGAC3360.12709412151862345No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3190.12066376417988356No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3110.11763771366753539No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3090.11688120103944834No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3070.11612468841136131No Hit
CCTACGGGGGGCTGCAGAAGCGGGATTAGATACCCCAGTAGTC3030.11461166315518721No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT3010.11385515052710017No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2880.10893781844453437No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC2770.10477699899005563No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGGAAGCCTGAC2770.10477699899005563No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2760.10439874267601212No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT2760.10439874267601212No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC2700.10212920479175099No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2670.10099443584962041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)