FastQCFastQC Report
Wed 8 May 2024
EGAF00002458449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264371
Total Bases71.3 Mbp
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG102543.8786402442022765No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA72022.7242019737414465No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG61622.3308154071361837No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG59432.247977274360652No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG54612.0656577309916746No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG47231.7865045712275551No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG41541.5712767285367912No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40201.5205903824549591No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39241.484277776306781No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG38081.4404000438777325No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG37601.4222437408036435No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG36971.3984135930189014No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG36901.395765798820597No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTACCTCAG36281.3723139073498984No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA35671.3492402721932437No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32301.2217678943605765No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31881.2058811291707487No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG28471.0768957260819076No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG27771.050417784098861No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA26561.0046487700995947No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG25720.972875239719939No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24890.9414799656543268No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG24210.9157585362993671No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24180.9146237673572366No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23160.8760416233247973No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG22190.8393507608625758No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA21660.819303176218269No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG20930.7916904652930918No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG20850.7886644147807438No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG20700.7829905700700909No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19960.7549996028308702No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19470.7364650434427377No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG18950.7167957151124745No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG17420.6589224990638156No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG16860.6377401454773785No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16500.6241229181718115No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG16060.6074796403538967No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG15960.6036970772134614No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG15960.6036970772134614No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG15950.6033188208994179No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG15800.597644976188765No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG15780.5968884635606779No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15340.580245185742763No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG14870.5624671389827174No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG14810.5601976010984564No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG14710.5564150379580212No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG13810.5223719696941042No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG13550.5125373055289726No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13450.5087547423885374No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG13330.504215666620015No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG12920.4887071577442306No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG12900.4879506451161436No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12440.47055085467014157No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12270.46412049733140176No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11900.45012501371179137No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11740.44407291268709503No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA11470.43385999220792No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG11350.4293209164393977No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11220.42440358435683184No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG11200.42364707172874483No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10670.4035994870844381No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10620.4017082055142206No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG10550.3990604113159159No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG10510.3975473860597418No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10500.3971691297456983No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG10200.3858214403243926No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10140.3835519024401315No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9860.37296072564691285No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG9720.36766513725030353No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG9250.349887090490258No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9020.341187195267257No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG7770.2939051560118167No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG7490.2833139792185981No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG7430.281044441334337No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6910.26137511300407384No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG6640.2511621925248987No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG6610.25002742358276814No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6420.24284055361594123No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG6380.24132752835976712No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6160.23300588945080963No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG6130.2318711205086791No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6000.2269537884261133No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6000.2269537884261133No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG5970.22581901948398272No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG5880.22241471265759105No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5840.22090168740141694No Hit
GACTACTGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5810.21976691845928636No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG5750.21749738057502524No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5730.21674086794693823No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5730.21674086794693823No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5090.19253246384815279No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4840.18307605599706475No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG4800.18156303074089064No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG4730.17891523654258598No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG4670.17664569865832486No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4550.17210662288980258No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4270.16151544609658397No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4230.16000242084040986No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4100.1550850887578441No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4100.1550850887578441No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCATGTTTCAG4060.15357206350167No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3880.14676344984888662No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3870.14638519353484308No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG3870.14638519353484308No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3670.13882006725397264No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG3590.13579401674162447No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3540.13390273517140686No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3540.13390273517140686No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG3490.13201145360118924No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3480.13163319728714573No Hit
GACTACAGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3460.13087668465905866No Hit
GACTACTCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3430.1297419157169281No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3400.12860714677479754No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG3350.12671586520457992No Hit
GACTACTGGGGTATCTAATCCCGCTTCTGCAGCCCCCCGTAGG3260.12331155837818823No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG3200.1210420204939271No Hit
GACTACTAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3150.11915073892370949No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGTGCATGAG3140.11877248260966597No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3010.11385515052710017No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2970.1123421252709261No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2850.10780304950240382No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG2830.10704653687431678No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG2800.10591176793218621No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2790.10553351161814269No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2740.10364223004792508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)