FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249032
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT94423.7914806129332774No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT91423.6710141668540595No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC85663.4397185903819594No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC70592.8345754762440167No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT67912.726958784413248No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT67502.7104950367824214No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT65322.622956085964856No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT64292.5815959394776575No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC55982.2479038838382217No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC51112.0523466863696234No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT49181.9748466060586591No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT45751.8371133027080857No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC34971.4042372064634263No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28631.1496514504160107No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG27951.1223457226380544No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC25461.022358572392303No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23780.9548973625879404No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23480.9428507179800186No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23020.9243791962478718No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC20270.8139516206752545No Hit
CCTACGGGTGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG19620.7878505573580906No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC18430.7400655337466671No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16620.6673841112788719No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16610.6669825564586077No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16610.6669825564586077No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16540.6641716727167594No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT16520.6633685630762312No Hit
CCTACGGGAGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG14960.6007260111150374No Hit
CCTACGGGCGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG14840.5959073532718686No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13140.5276430338269781No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT12980.5212181567027531No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12820.514793279578528No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12240.49150310000321246No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12170.48869221626136394No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11490.46138648848340774No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT10190.40918436184907964No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9980.4007517106235343No Hit
CCTACGGGGGGCTGCAGTAGTCAGTGGATTAGATACCCTAGTAGTCAGCA9310.3738475376658421No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT9290.373044428025314No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC8560.34373092614603745No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8060.3236531851328343No Hit
CCTACGGGGGGCAGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG7600.3051816634006875No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAGTC7050.28309614828616403No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7040.2826945934658999No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT6980.28028526454431557No Hit
CCTACGGGTGGCTGCAGTAGTCAGTGGATTAGATACCCTAGTAGTCAGCA6820.27386038742009056No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6630.2662308458350734No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6020.24173600179896562No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC5910.2373188987760609No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC5580.22406758970734686No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5470.21965048668444215No Hit
CCTACGGGTGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAGTC5430.2180442674033859No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5290.21242249991968903No Hit
CCTACGGGAGGCTGCAGTAGTCAGTGGATTAGATACCCTAGTAGTCAGCA5130.20599762279546402No Hit
CCTACGGGCGGCTGCAGTAGTCAGTGGATTAGATACCCTAGTAGTCAGCA4910.19716341674965465No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4910.19716341674965465No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4790.19234475890648592No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4400.1766841209161875No Hit
CCTACGGGCGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAGTC4360.17507790163513123No Hit
CCTACGGGAGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAGTC4290.1722670178932828No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4260.1710623534324906No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4250.17066079861222655No Hit
CCTACGGGTGGCAGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG4120.16544058594879374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980.15981881846509685No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC3680.14777217385717498No Hit
CCTACGGGGGGCTGCATACTGAGGCTGTGCAAGGCAGTAGGGCTCTGGGC3650.1465675093963828No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC3650.1465675093963828No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCTGTAGTC3570.1433550708342703No Hit
CCTACGGGGGGCTGCAGTCGAGAATTTTTCTCAATGGGCGCAAGCCTGAA3560.14295351601400622No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT3560.14295351601400622No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC3550.14255196119374217No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC3470.13933952263162966No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3450.13853641299110153No Hit
CCTACGGGTGGCTGCAGTCGAGAATTTTTCTCAATGGGCGCAAGCCTGAA3280.13170998104661247No Hit
CCTACGGGTGGCTGCATACTGAGGCTGTGCAAGGCAGTAGGGCTCTGGGC3210.12889909730476407No Hit
CCTACGGGTGGCTGCAGCCTTTATCTCCTGTAGCAGGGAGAAGAGAATAG3160.12689132320344373No Hit
CCTACGGGAGGCAGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG3110.12488354910212343No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC3030.12167111054001092No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3020.12126955571974687No Hit
CCTACGGGGGGCTGCAGCCTTTATCTCCTGTAGCAGGGAGAAGAGAATAG2970.11926178161842656No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCTAGTAGTC2860.11484467859552187No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC2770.1112306852131453No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2720.10922291111182499No Hit
CCTACGGGGGGCAGCAGTAGTCAGTGGATTAGATACCCTAGTAGTCAGCA2720.10922291111182499No Hit
CCTACGGGCGGCAGCAGGATGAAATGAGGCTGGATTAGATACCCCAGTAG2710.10882135629156092No Hit
CCTACGGGTGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCTGTAGTC2670.10721513701050468No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCTGTAGTC2630.10560891772944843No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCCGGTAGTC2600.10440425326865624No Hit
CCTACGGGGGGCTGCAGGATGAAATGAGGCTGGATTAGATACCCGAGTAGTC2540.10199492434707187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)