FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249032
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG96803.8870506601561243No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA89723.602749847409168No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG74652.997606733271226No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG57482.308137106877831No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG52512.108564361206592No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50622.032670500176684No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50622.032670500176684No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG49201.9756497156991872No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA44341.780494073050853No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG43771.7576054482958015No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG40511.6266985768897169No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG39831.5993928491117606No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39701.594172636448328No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG39071.568874682771692No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG34361.3797423624273186No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32911.3215169134890297No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32681.312281152622956No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31671.2717241157762857No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31241.2544572585049312No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG31161.2512448199428186No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31041.2464261620996497No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG30631.2299624144688233No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA28571.1472421214944264No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG25471.022760127212567No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG25331.0171383597288701No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG25321.016736804908606No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG23270.9344180667544734No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG19780.7942754344823156No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA17600.70673648366475No Hit
GACTACTGGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT13330.5352725754119952No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11660.4682129204278968No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11570.4645989270455202No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10290.4131999100517203No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10010.4019563750843265No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9860.39593305278036556No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9770.39231905939798906No Hit
GACTACTGGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT9170.36822577018214525No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8450.3393138231231328No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8290.3328889459989078No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7940.3188345272896656No Hit
GACTACTGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCCCCCGTAGG7720.3100003212438562No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7520.30196922483857497No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7180.28831636094959684No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7030.28229303864563593No Hit
GACTACTGGGGTATCTAATCCAACTACTGCTGACTACTGGGGTATCTAAT6770.2718526133187703No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6670.26783706511612965No Hit
GACTACAGGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT6640.26663240065533744No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG6570.26382151691348904No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6500.26101063317164064No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6290.25257798194609526No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5900.23691734395579686No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG5850.23490956985447653No Hit
GACTACTGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCACCCGTAGG5840.23450801503421248No Hit
GACTACTGGGGTATCTAATCCGACTACTGCTGACTACTAGGGTATCTAAT5600.22487069934787499No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5300.2128240547399531No Hit
GACTACAGGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT4980.1999743004915031No Hit
GACTACTCGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT4780.19194320408622184No Hit
GACTACTAGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT4580.1839121076809406No Hit
GACTACTGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCTCCCGTAGG4510.18110122393909217No Hit
GACTACTGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCGCCCGTAGG4490.18029811429856404No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4390.1762825660959234No Hit
GACTACAGGGGTATCTAATCCAACTACTGCTGACTACTGGGGTATCTAAT4240.17025924379196247No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4220.16945613415143435No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG4120.16544058594879374No Hit
GACTACCGGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT4120.16544058594879374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3980.15981881846509685No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG3970.1594172636448328No Hit
GACTACAGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCCCCCGTAGG3930.15781104436377655No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG3920.15740948954351247No Hit
GACTACTCGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT3880.15580327026245622No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG3870.15540171544219217No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3860.1550001606219281No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3850.15459860580166404No Hit
GACTACTAGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT3830.15379549616113591No Hit
GACTACCGGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT3820.15339394134087186No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGTGCCTCAG3740.15018150277875936No Hit
GACTACTGGGGTATCTAATCTTGACATAAGGCAGAGGATCTACTGAACTT3370.13532397442898902No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG3360.13492241960872497No Hit
GACTACACGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT3330.1337177551479328No Hit
GACTACAGGGGTATCTAATCCGACTACTGCTGACTACTAGGGTATCTAAT3290.13211153586687654No Hit
GACTACTGGGGTATCTGGAGGAAGAAATTTCTAAGCAGCAAAGCATTCAT3260.13090687140608437No Hit
GACTACTAGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCCCCCGTAGG3110.12488354910212343No Hit
GACTACCCGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT3090.1240804394615953No Hit
GACTACCAGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT3000.12046644607921875No Hit
GACTACTGGGGTATCTAATCCGACTACTGCTGACTACCGGGGTATCTAAT2990.12006489125895468No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG2960.1188602267981625No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG2890.11604934305631405No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2880.11564778823604999No Hit
GACTACAGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCACCCGTAGG2880.11564778823604999No Hit
GACTACAAGGGTATCTAATCCGACTACTGCTGACTACTGGGGTATCTAAT2870.11524623341578594No Hit
GACTACCGGGGTATCTAATCCGACTACTGCTGACTACTAGGGTATCTAAT2860.11484467859552187No Hit
GACTACCGGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCCCCCGTAGG2850.11444312377525781No Hit
GACTACTCGGGTATCTAATCCAGCCTCATTTCATCCTGCAGCCCCCCGTAGG2850.11444312377525781No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2780.11163224003340937No Hit
GACTACTGGGGTATCTAATCCGACTACTGCTGCAGCCCCCCGTAGG2730.10962446593208905No Hit
GACTACTCGGGTATCTAATCCGACTACTGCTGACTACTAGGGTATCTAAT2720.10922291111182499No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2660.10681358219024062No Hit
GACTACTCGGGTATCTAATCCAACTACTGCTGACTACTGGGGTATCTAAT2640.10601047254971249No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCACCCGTAGG2630.10560891772944843No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG2610.1048058080889203No Hit
GACTACACGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT2590.10400269844839218No Hit
GACTACTAGGGTATCTAATCCGACTACTGCTGACTACTAGGGTATCTAAT2560.10279803398759998No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2550.10239647916733594No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG2550.10239647916733594No Hit
GACTACCCGGGTATCTAATCCGACTACTGCTGACTACAGGGGTATCTAAT2530.10159336952680781No Hit
GACTACCCGGGTATCTAATCCTGCAGCCCCCCGTAGG2520.10119181470654375No Hit
GACTACTGGGGTATCTAATCCAGCCTCATTTCATCCTGCTGCCCCCCGTAGG2500.10038870506601562No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2500.10038870506601562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)