FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278395
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC161825.812604393038668No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC145895.240395840442537No Hit
CCTACGGGGGGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT113834.088794698180643No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC112924.056107329513821No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC98963.554661542053557No Hit
CCTACGGGTGGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT98633.5428078808886654No Hit
CCTACGGGAGGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT71812.5794285098511107No Hit
CCTACGGGCGGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT71302.561109215323551No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT60422.1702975987356097No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT58952.1174949262738196No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC54281.9497476606979294No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT41451.4888916826810827No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT40131.4414770380215163No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC36081.2960002873614829No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC33911.2180534851559834No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC33351.1979381813610157No Hit
CCTACGGGGGGCAGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT32051.1512419404084124No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27750.99678514341134No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25540.9174015337919142No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24290.8725013021067188No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC22830.8200578314984105No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC22000.7902440776594407No Hit
CCTACGGGTGGCAGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT20680.7428294329998743No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT18470.6634458233804487No Hit
CCTACGGGAGGCAGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT16550.5944790675119884No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT16380.5883726360028018No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC15480.556044469189461No Hit
CCTACGGGCGGCAGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT14720.5287451283248622No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT14610.524793907936565No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC12790.4594191706029203No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12070.4335566371522477No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC11710.42062537042691145No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT11000.39512203882972036No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT10750.3861419924926813No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC10580.38003556098349467No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC10180.3656674868442321No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9880.3548914312397852No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9450.33944575154007794No Hit
CCTACGGGGTGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT9410.33800894412615173No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8980.32256326442644445No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8780.3153792273568132No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC8530.3063991810197741No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8060.28951669390614054No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8010.28772068463873274No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7470.2683237845507283No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7450.2676053808437652No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7230.25970294006717076No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC6890.24749007704879758No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6840.24569406778138975No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6180.22198674545160654No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6160.2212683417446434No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5960.2140843046750121No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC5800.2083370750193071No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC5620.20187144165663895No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5180.18606656010345013No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC5180.18606656010345013No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5170.18570735824996856No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5050.1813969360081898No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC4850.17421289893855854No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC4750.1706208804037429No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4640.16666966001544567No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT4460.16020402665277753No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4350.15625280626448032No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTAGTAGTC4330.1555344025575172No Hit
CCTACGGGGGGCTGCAGTGTCCTGAGGCTGCACACAGCAGTGGAGCCATG4260.15301998958314625No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC4220.15158318216922No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT4190.1505055766087753No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4180.15014637475529372No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4150.14906876919484904No Hit
CCTACGGGGGGCTGCAGTGCAAGGGACTGGGATTAGATACCCCAGTAGTC4050.1454767506600334No Hit
CCTACGGGTGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT3930.14116632841825466No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3880.13937031915084683No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCTGTAGTC3810.1368559061764759No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3800.1364967043229943No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGAGTAGTC3760.13505989690906806No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCGGTAGTC3600.12931266725336302No Hit
CCTACGGGTGGCTGCAGTGCAAGGGACTGGGATTAGATACCCCAGTAGTC3560.12787585983943678No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT3510.12607985057202895No Hit
CCTACGGGGAGCTGCAGACGAGAATATTCGACAATGGGCGAAAGCCTGAT3460.12428384130462114No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGTGTAGTC3440.123565437597658No Hit
CCTACGGGTGGCTGCAGTGTCCTGAGGCTGCACACAGCAGTGGAGCCATG3430.12320623574417644No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTGGTAGTC3380.12141022647676862No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGGGTAGTC3360.12069182276980549No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCTAGTAGTC3360.12069182276980549No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3240.11638140052802673No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3130.11243018013972952No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCTAGTAGTC3100.11135257457928482No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCTGTAGTC3070.11027496901884012No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT3050.10955656531187702No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGAGTAGTC3020.10847895975143232No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT2990.10740135419098763No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCGAGTAGTC2950.10596454677706135No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2940.1056053449235798No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTTGTAGTC2900.10416853750965355No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2880.10345013380269041No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2880.10345013380269041No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2870.10309093194920886No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2840.10201332638876416No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCGGTAGTC2820.10129492268180103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)