FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278395
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA154645.554697462238905No Hit
GACTACTGGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG108113.8833312379891884No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA88733.187198045941917No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA86193.0959607751576No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA83733.0075971192011353No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA70782.54243071894251No Hit
GACTACAGGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG65172.3409184791393525No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG61802.2198674545160655No Hit
GACTACTCGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG60622.1774816358052407No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA58372.0966612187718887No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA57662.0711578871746976No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56462.0280536647569103No Hit
GACTACTAGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG53921.936816393972593No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50701.8211533971515295No Hit
GACTACCGGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG47061.69040392248424No Hit
GACTACACGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG39921.4339337990984033No Hit
GACTACCAGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG36911.3258140412004527No Hit
GACTACCCGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG35921.2902530577057778No Hit
GACTACAAGGGTATCTAATCCCATTCGCGACCCACGCTTTCGTGTTTCAG35141.2622353131342157No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG34901.253614468650658No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG34221.2291887426139119No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG32651.1727940516173063No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG30041.079042367858618No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27750.99678514341134No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG26800.9626609673305914No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG23760.853463603872196No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG22160.7959913073151458No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG20760.7457030478277268No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG20730.7446254422672821No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG19410.6972107976077156No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG19280.6925411735124554No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG17170.6167495824278453No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG15980.5740045618635392No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG13910.4996497781928555No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA13820.4964169615115214No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG12470.4479247112915103No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG11270.4048204888737226No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG11120.3994324610714991No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG10790.3875787999066075No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9440.3390865496865964No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG9380.336931338565707No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG9330.33513532929829914No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG8700.31250561252896064No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG8290.29777833653621655No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8090.29059429946658527No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7590.27263420679250705No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7380.26509096786939423No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG7230.25970294006717076No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG6800.2442572603674635No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6570.23599561773738753No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG6200.22270514915856965No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG6010.21588031394241997No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG5990.2151619102354568No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG5970.21444350652849367No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG5870.21085148799367803No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5840.2097738824332334No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG5590.20079383609619425No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG5560.19971623053574955No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG5360.1925321934661183No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5230.18786256937085796No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5160.185348156396487No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5110.1835521471290792No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG5090.18283374342211606No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4950.17780491747337418No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4940.1774457156198926No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4890.17564970635248478No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG4840.17385369708507695No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG4630.16631045816196413No Hit
GACTACTAGGGTATCTAATCCTGCAGCCACCCGTAGG4560.16379604518759316No Hit
GACTACTGGGGTATCTAATCCCAGTCCCTTGCACTGCAGCCCCCCGTAGG4470.1605632285062591No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4450.15984482479929596No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG4310.15481599885055408No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4310.15481599885055408No Hit
GACTACTGGGGTATCTGGTGGAATGAACTTCTAAGCAGCAAAGCTCAAGA4090.14691355807395967No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG4060.14583595251351497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4030.14475834695307027No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG3990.143321539539144No Hit
GACTACCGGGGTATCTAATCCTGCAGCCACCCGTAGG3920.14080712656477307No Hit
GACTACACGGGTATCTAATCCTGCAGCCCCCCGTAGG3910.1404479247112915No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3850.13829271359040213No Hit
GACTACCAGGGTATCTAATCCTGCAGCCCCCCGTAGG3830.137574309883439No Hit
GACTACCCGGGTATCTAATCCTGCAGCCCCCCGTAGG3700.13290468578817866No Hit
GACTACTAGGGTATCTAATCCTGCAGCCGCCCGTAGG3630.13039027281380772No Hit
GACTACTGGGGTATCTAATCCCAGTCCCTTGCACTGCAGCCACCCGTAGG3620.13003107096032615No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3540.12715745613247365No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3500.12572064871854738No Hit
GACTACTAGGGTATCTAATCCTGCAGCCTCCCGTAGG3450.12392463945113956No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3440.123565437597658No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3360.12069182276980549No Hit
GACTACAGGGGTATCTAATCCTGCAGCCTCCCGTAGG3310.11889581350239767No Hit
GACTACAAGGGTATCTAATCCTGCAGCCCCCCGTAGG3210.11530379496758204No Hit
GACTACTCGGGTATCTAATCCTGCAGCCTCCCGTAGG3200.11494459311410046No Hit
GACTACTCGGGTATCTAATCCTGCAGCCGCCCGTAGG3150.11314858384669266No Hit
GACTACTGGGGTATCTAATCCTGCTGCCCCCCGTAGG3090.11099337272580327No Hit
GACTACCGGGGTATCTAATCCTGCAGCCTCCCGTAGG3050.10955656531187702No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3030.10883816160491389No Hit
GACTACCGGGGTATCTAATCCTGCAGCCGCCCGTAGG3020.10847895975143232No Hit
GACTACTGGGGTATCTAATCCACTGCAGCCCCCCGTAGG2980.10704215233750605No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2980.10704215233750605No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2930.10524614307009825No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2880.10345013380269041No Hit
GACTACAGGGGTATCTAATCCTGCAGCCGCCCGTAGG2870.10309093194920886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)