FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304302
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC148884.8925081005054185No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC134404.416665023562119No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC103313.3949826159538876No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT93023.0568317000874132No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT90942.9884785509132374No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC90692.9802630281759566No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT89402.937870930851588No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT78592.58263172769157No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT66712.1922300872159894No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT62712.0607817234194976No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT57861.9014005823162519No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT57441.88759850411762No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC47791.5704793264585837No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT44421.4597340799600396No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT42761.4051830089844957No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40511.331243304348969No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT39751.3062681152276359No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC30791.0118237803234944No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29730.9769899639174242No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT29520.9700889248181084No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29350.9645023693567574No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT28890.949385807520161No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT28180.9260537229462837No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC27490.9033788801913889No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT25780.8471847046683887No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT25030.8225381364565465No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT23100.7591143009247393No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC22510.7397256672647567No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22490.7390684254457743No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19720.6480404335167038No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT18490.6076200616492826No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC18460.6066341989208089No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC17550.5767296961571071No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT17370.570814519786265No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT16830.5530689906737386No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT15880.5218500042720718No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14020.4607265151067032No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13260.4357513259853698No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT12820.42129200596775573No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12670.41636269232538725No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12170.39993164685082583No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT12140.39894578412235215No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12140.39894578412235215No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC11090.36444058862577305No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT10060.3305926349481765No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9170.3013453740034571No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC8870.2914867467187202No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8860.291158125809229No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8610.28294260307194824No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8520.2799850148865272No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8180.2688119039638254No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7890.2592818975885798No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT7340.2412077475665622No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC7040.2313491202818253No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6960.22872015300589546No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6730.2211618720875972No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6480.21294634935031648No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6410.21064600298387787No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6210.20407358479405327No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6200.20374496388456204No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5890.19355771569033395No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5810.1909287484144041No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5340.17548356566831635No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5180.1702256311164567No Hit
CCTACGGGGGGCTGCACTTTTCTACACGACGTCTTCCGATCTGCATACTA5050.1659535592930707No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4620.15182286018494784No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4480.14722216745207065No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT4470.1468935465425794No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4440.14590768381410574No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4380.14393595835715836No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4290.1409783701717373No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT4220.13867802380529867No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4140.13604905652936886No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT4030.13243422652496534No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3910.12849077561107058No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3810.1252045665161583No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC3740.12290422014971968No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3720.12224697833073722No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC3580.11764628559786001No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT3490.11468869741243896No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3460.11370283468396528No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCCAGTAGTC3450.11337421377447404No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC3430.11271697195549157No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3350.11008800467956176No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC3320.10910214195108807No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3280.10778765831312315No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT3230.106144553765667No Hit
CCTACGGGGGGCTGCAGGTGGATTAGATACCCCAGTAGTC3190.10483007012770208No Hit
CCTACGGGTGGCTGCACTTTTCTACACGACGTCTTCCGATCTGCATACTA3160.1038442073992284No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT3100.10187248194228102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)