FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304302
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA136944.5001347345728915No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG90772.9828919954518867No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA81752.686475935090798No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG78222.570472754040394No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA77282.5395823885482187No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA72912.3959750511005513No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA64572.1219052125848665No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA54801.8008425840119353No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG53051.7433339248509705No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA52821.7357756439326724No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50971.674980775676795No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG50971.674980775676795No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG48951.6085993519595665No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA48301.5872389928426365No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG47341.5556913855314787No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45231.4863523736288293No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG41451.3621336698411446No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG40511.331243304348969No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG37861.2441587633337934No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG36991.2155687442080565No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG35921.1804063068924948No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG34351.128812824102372No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32821.0785338249502139No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32501.0680179558464946No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32201.0581593285617577No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28740.9444564938777924No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28640.9411702847828801No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG28420.933940624774073No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG26300.8642729919619325No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25980.8537571228582131No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23710.7791601764037043No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG23070.7581284381962655No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG22310.7331532490749322No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21940.7209942754237567No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21850.7180366872383356No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG21420.7039059881302128No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG20130.6615138908058442No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19490.6404821525984056No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG17360.5704858988767737No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16060.5277651806429139No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG15520.5100196515303875No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG15360.5047617169785279No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14940.4909596387798963No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14810.4866875669565103No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14420.47387135148635234No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG13910.4571116851022997No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG13880.456125822373826No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13840.4548113387358611No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13600.44692443690807154No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG13200.4337796005284224No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13090.43016477052401886No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG12960.42589269870063295No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG11990.39401647047998367No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG11620.3818574968288082No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG11320.37199886954407135No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA10560.34702368042273796No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG9930.3263205631247905No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG9580.31481883129259747No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG9210.302659857641422No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG8680.28524294943838685No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG8430.27702742670110614No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG8420.2766988057916149No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG8290.272426733968229No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG7640.25106637485129907No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6420.2109746238933691No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6250.20538806843201818No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5820.19125736932389534No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5310.17449770293984265No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4910.1613528665601935No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4810.1580666574652812No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4620.15182286018494784No Hit
GACTACTGGGGTATCTAATCCGGTTACAGACAGTAGTATGCAGATCGGAA4320.14196423290021096No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4210.13834940289580744No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4070.13374871016293025No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3860.12684767106361441No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG3820.12553318742564953No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3780.1242187037876846No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG3750.12323284105921091No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3550.11666042286938633No Hit
GACTACTGGGGTATCTAATCCACCTGCAGCCCCCCGTAGG3540.11633180195989512No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3460.11370283468396528No Hit
GACTACTGGGGTATCTAATCCTTCGCTTCTGCAGCCCCCCGTAGG3360.11041662558905299No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3330.10943076286057929No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3230.106144553765667No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3200.10515869103719332No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3190.10483007012770208No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG3100.10187248194228102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)