FastQCFastQC Report
Wed 8 May 2024
EGAF00002458456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256625
Total Bases66.3 Mbp
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC139365.430491962981003No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC115964.518655625913297No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC87963.4275694106186068No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC82713.2229907452508524No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT58502.279590842669265No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT56982.220360448124696No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC41991.6362396492937163No Hit
CCTACGGGGGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC40291.5699951290793959No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT39051.521675596687774No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT38951.5177788602045785No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT35891.3985387238188018No Hit
CCTACGGGTGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC35131.3689235265465174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT30801.2001948368241597No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT29201.1378470530930347No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28341.1043351193375548No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT27071.054846566000974No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT26681.0396492937165123No Hit
CCTACGGGCGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC25570.9963955187530442No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT25240.9835362883584997No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC24850.9683390160740379No Hit
CCTACGGGAGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC24770.9652216268874818No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT22450.8748173404773503No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT21750.847540185094983No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20400.7949342425718461No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT19750.7696054554310765No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT19440.757525572333171No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19290.7516804676083779No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT18570.7236239649293716No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC17480.6811495372625426No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT17290.6737457379444715No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16530.644130540672187No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC14000.5455431076473454No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13800.5377496346809547No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13040.5081344374086703No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC12500.4870920603994155No Hit
CCTACGGGGGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC12380.48241597661958113No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10940.4263029712615684No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC10930.42591329761324886No Hit
CCTACGGGGGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAG9890.38538723818801757No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9600.3740867023867511No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9520.37096931320019483No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG9330.3635655138821237No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9100.3546030199707745No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT8900.3468095470043838No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT8780.34213346322454946No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8400.32732586458840723No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC8220.3203117389186556No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8210.3199220652703361No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC7790.3035557720409157No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7770.3027764247442767No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7550.29420360448124694No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7430.2895275207014126No Hit
CCTACGGGTGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC7410.2887481734047735No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7400.288358499756454No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT7320.2852411105698977No Hit
CCTACGGGTGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAG7220.2813443740867024No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6970.27160253287871405No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG6920.26965416463711644No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT6870.2677057963955187No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC6250.24354603019970775No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6080.2369215781782757No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA6010.23419386264003894No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5840.22756941061860692No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5800.2260107160253288No Hit
CCTACGGGAGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC5720.22289332683877255No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5710.222503653190453No Hit
CCTACGGGAGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAG5630.21938626400389677No Hit
CCTACGGGCGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAG5500.2143205065757428No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT5450.21237213833414514No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC5330.20769605455431078No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5310.2069167072576717No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5280.2057476863127131No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5240.204188991719435No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5220.2034096444227959No Hit
CCTACGGGGGGCTGCTTTATGGATTAGATACCCCAGTAGTC5200.20263029712615682No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA5170.20146127618119827No Hit
CCTACGGGCGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC5080.19795421334632243No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5060.1971748660496834No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5030.1960058451047248No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT4880.1901607403799318No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4850.18899171943497323No Hit
CCTACGGGTGGCTGCAGTCGAGAATATTCCTCAATGGACGAAAGTCTGAA4660.18158792011690209No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4540.17691183633706772No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4530.17652216268874818No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4420.1722357525572333No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4410.1718460789089138No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC4400.17145640526059427No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT4200.16366293229420362No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC4150.16171456405260595No Hit
CCTACGGGTGGCTGCTTTATGGATTAGATACCCCAGTAGTC4150.16171456405260595No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4120.16054554310764735No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT4030.15703848027277154No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4000.15586945932781296No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3960.15431076473453484No Hit
CCTACGGGGGGCTGCAGTCGAGAATATTCCTCAATGGACGAAAGTCTGAA3900.15197272284461763No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA3800.1480759863614223No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC3670.1430102289332684No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3660.14262055528494885No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3640.1418412079883098No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3440.13404773502191913No Hit
CCTACGGGCGGCTGCTTTATGGATTAGATACCCCAGTAGTC3390.13209936678032147No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA3310.12898197759376523No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT3300.12859230394544569No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3270.1274232830004871No Hit
CCTACGGGAGGCTGCTTTATGGATTAGATACCCCAGTAGTC3190.12430589381393084No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3160.12313687286897224No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3100.12079883097905503No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC3100.12079883097905503No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3090.12040915733073551No Hit
CCTACGGGGTGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC3090.12040915733073551No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT3070.11962981003409644No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3060.11924013638577691No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3050.11885046273745739No Hit
CCTACGGGGGGCTGCTTTATGGATTAGATACCCGAGTAGTC3010.11729176814417924No Hit
CCTACGGGGGGCTGCTTTATGGATTAGATACCCCTGTAGTC2960.11534339990258158No Hit
CCTACGGGGGGCTGCAGAAGCGAGGAGGATTAGATACCCCAGTAGTC2930.11417437895762299No Hit
CCTACGGGAGGCTGCAGTCGAGAATATTCCTCAATGGACGAAAGTCTGAA2900.1130053580126644No Hit
CCTACGGGCGGCTGCAGTCGAGAATATTCCTCAATGGACGAAAGTCTGAA2900.1130053580126644No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT2800.10910862152946907No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT2760.10754992693619095No Hit
CCTACGGGGGGCTGCTTTATGGATTAGATACCCTAGTAGTC2740.10677057963955187No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCGCAATGGGCGAAAGCCTGAC2730.10638090599123233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)