FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256625
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA133195.190063321967852No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA78583.0620555284948856No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA73782.87501217730151No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA69332.701607403799318No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA58802.2912810521188502No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG58222.2686799805163176No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA51011.9877252800779348No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46891.8271797369702876No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46551.8139308329274233No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA43461.693521675596688No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG36821.4347783731125183No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG35201.3716512420847542No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG33871.3198246468582562No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG30591.1920116902094495No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG29051.1320019483682415No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG27561.0739405747686313No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG26381.0279590842669264No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG22270.8678032148075986No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG21230.8272771553823672No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG21200.8261081344374086No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG20930.8155869459327814No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG20810.8109108621529468No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG19250.7501217730150999No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG18510.7212859230394545No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18290.7127131027764247No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG17490.6815392109108621No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG16760.6530930345835363No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG16370.6378957622990745No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG16140.6289332683877252No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG15840.6172430589381394No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG15470.6028251339503166No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG15160.5907452508524111No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA15110.5887968826108134No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG14900.5806137359961032No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG14090.5490501704822212No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG13880.540867023867511No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG13350.5202143205065757No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG12920.5034583536288358No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG12470.48592303945445686No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCTGGCTTTCGTGTTTGAG12190.47501217730151No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11760.4582562104237701No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG11280.4395518753044325No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11040.4301997077447638No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10660.41539210910862157No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10490.4087676570871895No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG10480.40837798343887No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG10370.40409157330735507No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG10100.3935703848027277No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9950.3877252800779347No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG9930.38694593278129563No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG9690.3775937652216269No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG9680.37720409157330737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9550.3721383341451534No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG9090.35421334632245494No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG9090.35421334632245494No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9070.3534339990258159No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8640.33667803214807596No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8640.33667803214807596No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8280.3226497808085728No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8210.3199220652703361No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7660.2984900146127618No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG7580.29537262542620557No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7380.2875791524598149No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7340.2860204578665368No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7100.276668290306868No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7070.2754992693619094No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6990.27238188017535314No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6870.2677057963955187No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6780.26419873356064294No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG6650.25913297613248903No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6600.25718460789089137No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG6250.24354603019970775No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6110.23809059912323427No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6050.2357525572333171No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6050.2357525572333171No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG5960.2322454943984413No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5950.23185582075012176No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5710.222503653190453No Hit
GACTACTGGGGTATCTAATCCATAAAGCAGCCCCCCGTAGG5660.22055528494885532No Hit
GACTACTGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5470.2131514856307842No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5330.20769605455431078No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG5300.20652703360935215No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5190.2022406234778373No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5010.1952264978080857No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4960.19327812956648807No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4960.19327812956648807No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4890.19055041402825135No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4690.1827569410618607No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4590.17886020457866536No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG4500.17535314174378958No Hit
GACTACTGGGGTATCTAATCCATAAAGCAGCCACCCGTAGG4460.17379444715051146No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4460.17379444715051146No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4420.1722357525572333No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4400.17145640526059427No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCATGAG4330.16872868972235752No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4280.16678032148075986No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4270.1663906478324403No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4140.1613248904042864No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG3890.1515830491962981No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG3760.14651729176814418No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG3720.14495859717486603No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG3620.14106186069167073No Hit
GACTACTGGGGTATCTAATCCATAAAGCAGCCGCCCGTAGG3550.138334145153434No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3540.13794447150511446No Hit
GACTACTGGGGTATCTAATCCATAAAGCAGCCTCCCGTAGG3370.13132001948368244No Hit
GACTACTCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3370.13132001948368244No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3320.12937165124208477No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG3290.12820263029712614No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3270.1274232830004871No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3270.1274232830004871No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3250.12664393570384802No Hit
GACTACAGGGGTATCTAATCCATAAAGCAGCCCCCCGTAGG3240.12625426205552848No Hit
GACTACTAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3200.12469556746225036No Hit
GACTACTCGGGTATCTAATCCATAAAGCAGCCCCCCGTAGG3190.12430589381393084No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3180.12391622016561131No Hit
GACTACTAGGGTATCTAATCCATAAAGCAGCCCCCCGTAGG3160.12313687286897224No Hit
GACTACTGGGGTATCTAATCCTCCTCGCTTCTGCAGCCCCCCGTAGG3160.12313687286897224No Hit
GACTACAGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3140.12235752557233318No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3080.12001948368241598No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCAAGCTTTCATGTTTCAG3030.11807111544081832No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3000.11690209449585973No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2970.11573307355090112No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2970.11573307355090112No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2930.11417437895762299No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCATGAG2910.11339503166098394No Hit
GACTACCGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG2900.1130053580126644No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2890.11261568436434487No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2840.1106673161227472No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2830.11027764247442766No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2810.10949829517778861No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2800.10910862152946907No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2760.10754992693619095No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2690.10482221139795421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)