FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772545
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG13961418.07195697338019No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG13561617.554446666537224No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA8218010.637568038107812No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC7785610.077859542162592No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA406985.268042638292915No Hit
TCCGTAGGTGAACCTGCGGAAAGATCATTACAGAGTTCATGCCCGAAAGG394405.105204227585449No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG326134.22150166009747No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATACAGATGCCTGTCG258843.35048443779974No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTCATTATGTTATTCT192192.4877515225650284No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG57410.7431282320123747No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC22340.28917409341850636No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA21470.27791261350471497No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC17910.23183115546667182No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA14210.18393750525859334No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG14190.18367862066287402No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG13280.1718993715576439No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGAGGGCCCTCTGGG13160.17034606398332783No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG13140.1700871793876085No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGATGGCCCTCTGGG10370.13423166288047947No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG10030.1298306247532506No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG10000.1294422978596716No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGATGCCTAAACATCAAAC8610.11144981845717726No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTATTGATTTGACTATACTTTTG8330.10782543411710646No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGCCCTCTGGG8290.10730766492566776No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTCACACATCCAATCAAACCTAA8070.10445993437275498No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCACACCTAA7830.10135331922412286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)