FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772545
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT13881017.967885365901015No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13269617.17647515678698No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT8115010.50424247131235No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG7945510.284837776440208No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT7759510.044075102421218No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG436235.646661359532454No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT277823.5961659191373965No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT203392.6327268961678607No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA161732.0934702832844687No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT74400.9630506960759568No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG24380.31558032218187937No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA23850.3087198803953168No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTCGAAAGTTT19990.25875515342148353No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA17900.23170171316881216No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG14660.18976240866227856No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG14100.18251363998213696No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11860.15351856526157054No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA10970.14199820075205974No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGCTGAAAGTTT9950.12879508637037324No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT9770.12646512500889914No Hit
GCTGCGTTCTTCATCGATCTGGGAGCCAAGACATCCACTGTTGAAAATTT9690.12542958662602177No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCG9130.11818081794588017No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGGTT8980.11623918347798509No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT8880.11494476049938838No Hit
GCTGCGTTCTTCATCGATACGGGAGCCAAGACATCCACTGTTGAAAATTT8770.11352089522293199No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAACGTTT8360.10821376101068546No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTG8070.10445993437275498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)