FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905022
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG19300321.32577992579186No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA640977.082369268371376No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC537975.944275387780628No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAACGGGTTGT525535.806820165697629No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAAATTCAAGGACGCTCT458645.067722110622725No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG415744.593700484629102No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTAAAAATAAAAAGG397384.390832488050014No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTTACC360283.9808977019343175No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA298113.2939530751738633No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA283883.136719328369918No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC264762.9254537458757905No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAAAGGGGTTG251362.77739104684748No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTCCGG200842.219172572600445No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGACTTCGG197942.1871291526614822No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCAAAACA130771.4449372501441955No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG27580.3047439730746877No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG20440.22585086329393098No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACAAGAACGCCCGGGCTTCGG20120.22231503764549368No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA19320.2134754735244005No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAATAATCAATAATCTTGGCT19100.21104459339109988No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC17270.1908240904640992No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCTAAAACTTTCCAC17060.18850370488231227No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG13740.1518195137797755No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGAACGCCCGGGCTTCGG11860.13104653809520653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)