FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002462818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905022
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT34303137.903056500283974No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG825209.118010390907624No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG602116.652987441189275No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG584936.463157801688799No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCCGAAAGTTT496085.481413711489886No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG444384.910156880164239No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA422214.665190459458444No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT376164.156363049738018No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA276123.050975556395314No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT29540.326400905171366No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCAAAAGTTT25030.2765678624387031No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA24160.2669548364570143No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG22500.24861274090574592No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG19340.21369646262742784No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT18320.20242601837303403No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT17830.19701178534886443No Hit
GCTGCGTTCTTCATCGATGTGCGAGCCTAGACATCCACTGCTGAAAGTTG17240.19049260680955823No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT15410.17027210388255754No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT13730.15170901922826185No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTT12320.1361292874648351No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTG10360.11447235536815678No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAATAGTTT10160.1122624643378835No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT9740.10762169317430957No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAACGTTT9710.10729020951976859No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGGTAAAAGTTT9660.10673773676220025No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG9160.10121300918651702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)