FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002462820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870008
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT21358124.54931448906217No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT16544219.016146977958822No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG9721411.173920239813887No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA838399.636578054454672No Hit
GCTGCGTTCTTCATCGACCCACGAGCCGAGTGATCCACCGTTCAGGGTTG681497.833146361872534No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT530066.092587654366397No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG28610.32884755082711886No Hit
GCTGCGTTCTTCATCGACCCCCGAGCCGAGTGATCCACCGTTCAGGGTTG26680.30666384676922515No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT25740.2958593484197847No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT21260.2443655690522386No Hit
GCTGCGTTCTTCATCGATCCACGAGCCGAGTGATCCACCGTTCAGGGTTG18930.21758420612224255No Hit
GCTGCGTTCTTCATCGACCCACGAGCCGAGTGATCCACCGTTCCGGGTTG17140.19700968266958466No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA15050.17298691506285No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG13900.15976864580555583No Hit
GCTGCGTTCTTCATCGATTCCAGAACCAAGAGATCCGTTGTTGAAAGTTT13510.15528592840525604No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG12470.14333201533779No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT12260.14091824442993628No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT11410.13114821932671883No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT11180.12850456547526No Hit
GCTGCGTTCTTCATCGACCCACGAGCCGAGTGATCCACCGTCCAGGGTTG11060.12712526781362932No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT10940.1257459701519986No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCAAAAGTTT10240.11770006712581954No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGATATCCATCGTTGAGAATTT9880.11356217414092744No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG9540.10965416409964045No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGTTCAGGGTTG9400.10804498349440464No Hit
GCTGCGTTCTTCATCGACCCACGAGCCGCGTGATCCACCGTTCAGGGTTG8830.10149331960165883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)