FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775680
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG22950529.5875876650165No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAG479606.18296204620462No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA414855.348210602310231No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC399565.151093234323432No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGTTCTCTCTT391535.047571163366336No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCAA351474.531121080858086No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTCT259563.3462252475247527No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA244923.157487623762376No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCAGAGTTCATGCCCGAAAGG211792.73037850660066No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG198492.5589160478547854No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG195172.516114892739274No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGAGTTCTAAAAGACTCCC136641.7615511551155114No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTTTGTGGGTTTTTGTATGGTA135351.744920585808581No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC113131.4584622524752475No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCG110271.4215913778877887No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATGCCTGGGCTGTCAT65710.8471276815181518No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGCAAGG41690.537463902640264No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT30980.39939150165016507No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACAGAGTTCATGCCCGAAAGG23850.30747215346534656No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA20870.2690542491749175No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTACTATACTCATAAC13870.1788108498349835No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGACAGG13720.17687706270627063No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAACGG8850.1140934405940594No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGGTCATGCCCGAAAGG8170.10532693894389439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)