FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775680
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT31905041.13165222772277No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT9087411.715398102310232No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG670438.643126031353136No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG412975.323973803630363No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT272903.5182033828382835No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT258573.3334622524752473No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA206952.6679816419141913No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT203352.6215707508250823No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCAGTTGTTGAAAGTTT151301.950546617161716No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATACGTTGTTGAAAGTTT90921.1721328382838283No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGTCGTCCATTATGAAGACTTG72860.9393048679867988No Hit
GCTGCGTTCTTCATCGATACAAGAGCCAAGTCGTCCATTATGAAGACTTG70100.9037231848184819No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT55370.7138252887788779No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG21730.28014129537953797No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT18860.24314150165016502No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG17030.219549298679868No Hit
GCTGCGTTCTTCATCGATGCTGGAGCCAAGAGATCCGTTGCTGAAAGTTT14680.18925330033003301No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT13120.16914191419141913No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTT10180.13123968646864687No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT9920.12788778877887788No Hit
GCTGCGTTCTTCACCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT8760.11293316831683167No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT8490.10945235148514852No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTGAAAGTTT8200.10571369636963697No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG8040.10365099009900991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)