FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002462823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796106
Sequences flagged as poor quality0
Sequence length35-301
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA42682853.61446842505897No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTCTGAGATTACTATCACATTT447635.62274370498401No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA223182.8033955277312317No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATCTGGAGCTTACTCG210412.642989752620882No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA127581.6025504141408304No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCAA115211.4471690955727001No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG77730.9763775175667563No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTCT66960.8410940251675029No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG64500.8101936174328543No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAATGAATGTAGAGTTGG54400.6833260897418183No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG54250.681441918538486No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACGTATCCACGTATCAAC49620.6232838340622983No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGAAACCTGCCCAGCAGAA44530.5593476245625583No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCCAAATTTTTCTACTTCGCCG41160.5170165781943611No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC40940.5142531270961405No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCCGCAGAA40240.5054603281472568No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACAAGGTCCAAAACCT28610.359374254182232No Hit
TCCGTAGGTGAACCTGTGGAAGGATCATTGTCTAAACCTGCCTAGCACAA27480.3451801644504626No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGCA26700.3353824741931351No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCAAAACA25200.31654076215981286No Hit
TCCGTAGGTGAACCTGCGGAAGGGTCATTGTCGAAACCTGCCCAGCAGAA21360.26830597935450806No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCACAGCAGAA20970.2634071342258443No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCCGAA20640.2592619575785134No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCCGAAACCTGCCCAGCAGAA19440.24418858795185566No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAAACCTGCCCAGCAGAA19240.24167635968074605No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTGTCGAAACCTGCCCAGCAGAA18930.2377824058605261No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTTCCCAGCAGAA17290.21718213403742717No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAC15580.19570258231943988No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC12950.1626667805543483No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCATCAGAA12940.1625411691407928No Hit
TCCGTAGGTGAACCTGCGGCAGGATCATTGTCGAAACCTGCCCAGCAGAA12640.15877282673412837No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACATCTCTGTTTTTGAAA12450.15638620987657423No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACTGATTTTGTCATGTTGGGT12430.15613498704946327No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCATAA10970.137795720670363No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG10230.12850047606725737No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA10160.127621196172369No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCTAAAACTTTCCAC9020.11330149502704415No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTGTCGAAACCTGCCCAGCAGAA8690.10915631837971325No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACGAAACCTGCCCAGCAGAA8490.10664409010860362No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTTGAAACCTGCCCAGCAGAA8430.10589042162727075No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG8190.10287574770193918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)