FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625270
Sequences flagged as poor quality0
Sequence length35-301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT24827439.7066867113407No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT7676312.276776432581125No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6407310.247253186623379No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG490287.841092647976073No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA360535.765989092711949No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATACATCGTTGAGAATTT183482.9344123338717676No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAGAGTTG80761.2916020279239369No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG35450.5669550754074241No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGACATCCATCGTTGAAAATTT34810.5567194971772194No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCATCGTTGAAAATTT31850.5093799478625234No Hit
GCTGCGTTCTTCATCGGTATGGGAACCGAGACATCCATCGTTGAAAATTT22960.3672013690085883No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT21810.3488093143761895No Hit
GCTGCGTTCTTCATCGTTCTGGGAACCTAGACATCCATCGTTGAAAATTT16550.26468565579669584No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG13530.21638652102291808No Hit
GCTGCGTTCTTCATCGTTACGGGAACCTAGACATCCATCGTTGAAAATTT13420.21462728101460168No Hit
GCTGCGTTCTTCATCGATATAGGAGCCTAGATATCCATCGTTGAAAATTT10880.17400482991347738No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAGTTT10290.16456890623250756No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT9310.14889567706750684No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT9040.1445775425016393No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTCGAAAATTT8380.13402210245174084No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT8380.13402210245174084No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT8180.13082348425480195No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA7880.12602555695939355No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAACTTT7770.12426631695107712No Hit
GCTGCGTTCTTCATCGTTATGAGAACCTAGACATCCATCGTTGAAAATTT7710.12330673149199545No Hit
GCTGCGTTCTTCATCGTTATCGGAGCCTAGATATCCATCGTTGAAAATTT7180.11483039327010731No Hit
GCTGCGTTCTTCACCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT6750.10795336414668863No Hit
GCTGCGTTCTTCATCGCTATGGGAACCTAGACATCCATCGTTGAAAATTT6430.10283557503158636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)