FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614956
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTCT13084321.276806795933368No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA11369018.48750154482597No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTGCATATCCAATCCAACCTAA380816.1924755592270015No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAA342565.570479839208009No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG301334.900025367668581No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA227833.7048179056713004No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCACAAACCTAA227013.6914836183401736No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC190043.0903023956185485No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG165582.692550361326664No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAATATCAATACAACACTGC161332.623439725768998No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAATAAACCTGGTGGATT89111.4490467610690847No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCATATCAACCTAA58940.9584425552397244No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC45840.7454191844619777No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA37960.6172799354750583No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAAATGAAATACCTT27710.45060134383598177No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACAACAAAATGAACTTTTCT27250.4431211338697403No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG21000.34148784628493745No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGCCACTTATAAACCTGTC18210.2961187467070815No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAACAAAATGAACTTTTCT12810.20830758623381188No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTTACAACAAAATGAACTTTTCT12580.2045674812506911No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGTAATTTTATCTGACTAG10210.16602813859853388No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC9600.15610872973025713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9490.15431998386876458No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAACATGAACTTTTCT9250.15041726562550817No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA8280.13464377939234679No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA8140.1323671937504472No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA7110.11561802795647168No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTCTTCT6910.112365762753758No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAACTGAACTTTTCT6760.10992656385172273No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA6340.10309680692602398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)