FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614956
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT13853222.527140153116644No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG12655020.578708070170872No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT388036.309882333044965No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG355005.7727707348168No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA315155.124756893176097No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT242583.944672464371435No Hit
GCTGCGTTCTTCATCGATGCATAAGCCGTGTGCTCCATTGCACAGAGTTT200513.2605584789806104No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT189173.076155041986744No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT175322.8509356766988208No Hit
GCTGCGTTCTTCATCGTTACGGGAACCAAGATATCCATCGTTGAGAATTT156942.5520525045694327No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG100511.634425877623765No Hit
GCTGCGTTCTTCATCGTTACGAGAGCCGAGATATCCATCGCTGAGGTTTT62751.0203982073514202No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCATCGTTGAGAATTT49230.8005450796479747No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG35090.5706099298161169No Hit
GCTGCGTTCTTCATCGATACGGGAACCAAGATATCCATCGTTGAGAATTT28590.464911310727922No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCACCGTTGAAAATTT27290.44377158691028296No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT24840.4039313381770403No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCACCGTTGAAAATTT18520.30115975777128773No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGACATCCATCGTTGAAAATTT18340.29823271908884535No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCATCGTTGAAAATTT16170.2629456416394018No Hit
GCTGCGTTCTTCATCGGTATGGGAACCGAGACATCCATCGTTGAAAATTT15250.2479852217069189No Hit
GCTGCGTTCTTCATCGTTATGAGAACCGAGATATCCACCGTTGAAAATTT10170.16537768555799115No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC9590.15594611647012144No Hit
GCTGCGTTCTTCATCGTTCTGGGAACCTAGACATCCATCGTTGAAAATTT9420.15318169104781482No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9290.1510677186660509No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT9220.14992942584510113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9100.1479780667234729No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG7990.12992799484841192No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA7420.1206590390206779No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT7190.11691893403755717No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG7100.115455414696336No Hit
GCTGCGTTCTTCATCGTTACGGGAACCTAGACATCCATCGTTGAAAATTT6510.10586123234833061No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG6210.10098283454426007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)