FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640520
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACAATGAACTTATAA13113820.47367763692No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACACCTAATCCTATATACCC7745012.09173796290514No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG638789.972834571910322No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACAACCTTTTCTACCGTG588909.194092300006245No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTTTGTGGGTTTTTGTATGGTA505737.895616061949666No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG447126.98057828014738No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA310654.849965652907013No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT105861.6527196652719665No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT61380.9582838943358521No Hit
TCCGTAGGTGAACCTGCGGAAAGACCCCTTGGGGGGATCGCCCGTCCGCC23060.3600199837631924No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18580.2900768125897708No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAATTTATTAATGTTTGTT16210.25307562605383127No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT15750.24589396115656031No Hit
TCCGTAGGTGAACCTGCGGGCGCCTGTGGGGTGGTGTCCGCGCTCGCCCC15150.2365265721601199No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAATAAAACAATGAACTTATAA14380.22450508961468807No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACACTGAACTTATAA9070.1416036969961906No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGACACCTAATCCTATATACCC8760.13676387934802972No Hit
TCCGTAGGTGAACCCCCGGGCGCCTGTGGGGTGGTGTCCGCGCTCGCCCC8470.13223630799975022No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACAATGAACTCATAA8010.12505464310247924No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCACAACCTTTTCTACCGTG7340.11459439205645412No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG6960.10866171235870854No Hit
TCCGTAGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGATGAAGAACGCAGC6940.1083494660588272No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACCATGAACTTATAA6680.10429026416036971No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACCTAATCCTATATACCC6510.10163617061137827No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG6450.10069943171173422No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACAATGAACTTCTAA6440.10054330856179355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)