FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640520
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGTTATGGGAGCCTAGACATCCATCGTTGAAAATTT9829915.346749516018235No Hit
GCTGCGTTCTTCATCGATGCATGAGCCAAGATATCCACTGTTAAGAGTTG7927612.37681883469681No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA626959.788140885530506No Hit
GCTGCGTTCTTCATCGATACAAGAGCCAAGTCGTCCATTATGAAGACTTG409906.399487916068194No Hit
GCTGCGTTCTTCATCGTTATGGGAGCCTAGACATCCATAGTTGAAAATTT348405.439330543933055No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT319144.982514207206645No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGACATCCGTTGCTGAAAGTGT311124.857303440954225No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGACATCCGTTGCTGAAAGTGT288954.511178417535752No Hit
GCTGCGTTCTTCATCGATGCGGGGTGGGTGTGCGGAGGGAAGCGCGCGGC172042.6859426715793417No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGTCGTCCATTATGAAGACTTG123081.9215637294698058No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG106791.667239118216449No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG91851.4339911322050833No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG58420.9120714419534128No Hit
GCTGCGTTCTTCATCGACGCGGGGTGGGTGTGCGGAGGGAAGCGCGCGGC57400.8961468806594642No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA54310.8479048273277963No Hit
GCTGCGTTCTTCATCGATATGGGAGCCTAGACATCCATCGTTGAAAATTT25860.4037344657465809No Hit
GCTGCGTTCTTCATCGGTATGGGAGCCTAGACATCCATCGTTGAAAATTT19610.30615749703366013No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17780.277586960594517No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT17220.26884406419783924No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGACATCCGTTGCTGAAAGTGT15430.24089802035845873No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA14250.22247548866545933No Hit
GCTGCGTTCTTCATCGATGCAGGAGCCAAGATATCCACTGTTAAGAGTTG13410.2093611440704428No Hit
GCTGCGTTCTTCATCGATACAAGAGCCAAGGCGTCCATTATGAAGACTTG12540.1957784300256042No Hit
GCTGCGTTCTTCATCGTTCTGGGAGCCTAGACATCCATCGTTGAAAATTT11230.17532629738337602No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG9970.15565478049085119No Hit
GCTGCGTTCTTCATCGATATGGGAGCCTAGACATCCATAGTTGAAAATTT8160.12739649035158934No Hit
GCTGCGTTCTTCATCGTTACGGGAGCCTAGACATCCATCGTTGAAAATTT7330.11443826890651346No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA7260.11334540685692875No Hit
GCTGCGTTCTTCATCGATGCCTGAGCCAAGATATCCACTGTTAAGAGTTG7130.1113158059077No Hit
GCTGCGTTCTTCATCGGTGTGGGAACCAAGACATCCGTTGCTGAAAGTGT6790.1060076188097171No Hit
GCTGCGTTCTTCATCGATGCACGAGCCAAGATATCCACTGTTAAGAGTTG6550.10226066321114095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)