FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735236
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA20801028.291596167761103No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG9315412.669945432486983No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTAT698119.495046488474449No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG421065.72686865169823No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGGGTTCATGCCCGAAAGG366294.981937772361528No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGTGACCCTGACCAAAACA266443.623870430718844No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTCTAA208442.8350080790385674No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA123261.6764685080708779No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG111441.5157038012284492No Hit
TCCGTAGGTGAACCTGCGGAATGATCATTTACAACAAAATGAACTTTTAT81791.11243192661948No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACGTCTTCTTGTAAACAA71390.970980746318189No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTTCGATGAAAAACCTTTTTTCA55950.7609801478708877No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATGAATTAATAATATTTGTGA52170.70956808426138No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA36360.4945350880533598No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCAATCCAAAACTTACAAAC35690.48542236778394965No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC23090.314048822418924No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC23030.3132327579171858No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA15840.2154410284588894No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA15750.21421693170628206No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACAACAAAATGAACTTTTAT14890.20252000718136762No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCCGGCCA14620.1988477169235456No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA13210.17967020113269752No Hit
TCCGTAGGTGAACCTGCGGGCATCGATGAAGAACGCAGC12880.17518184637313736No Hit
TCCGTAGGTGAACCTGCCCATGCATCGATGAAGAACGCAGC11520.15668438433373774No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGAGGGCCCTCTGGG9730.1323384600318809No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG9040.12295371826189143No Hit
TCCGTAGGTGAACCTGCAGAAGGAGCATTAGTGAAGATTTGGGCAGGCCA8600.11696924524914448No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGATGGCCCTCTGGG8130.11057673998552846No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG7880.10717647122828589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)