FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735236
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG22020429.950111256793736No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTAAAAATTT10456114.221420061041625No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT10000913.602299125722897No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT678269.225065149149389No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA441546.005418668291542No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG292483.9780424244732306No Hit
GCTGCGTTCTTCATCGGTATGGGAACCAAGATATCCATCGTTGAATATTT72280.9830857030939725No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCAAAAGTTG63320.8612200708343988No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG61940.8424505872944198No Hit
GCTGCGTTCTTCATCGATGCATGAGCCAAGAGATCCACCGCTAAAAGTTG37400.5086802060834889No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT37370.5082721738326197No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA24550.3339063919612206No Hit
GCTGCGTTCTTCATCGATGCATAAGCCGTGTGCTCCATTGCACAGAGTTT22050.2999037043887949No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT20040.2725655435805646No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT15340.20864049094440423No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGACATCCATCGTTAAAAATTT14470.19680755566920008No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT13810.1878308461500797No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG13470.1832064806402298No Hit
GCTGCGTTCTTCATCGATGCCCGCAGGTTCACCTACGGA13320.18116631938588426No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG13010.17694998612690346No Hit
GCTGCGTTCTTCATCGATGCATGGGCAGGTTCACCTACGGA12010.16334891109793317No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT11870.16144476059387733No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCATCGTTAAAAATTT11560.15722842733489656No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG11220.15260406182504666No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10060.13682681479144113No Hit
GCTGCGTTCTTCATCGGTATGGGAACCGAGACATCCATCGTTAAAAATTT9670.1315223955301427No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA8300.11288892274045341No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG7920.1077205142294447No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCGAAAGTTG7450.10132800896582865No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTG7420.10091997671495954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)