FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683167
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG11538916.890306469721168No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA526997.713926463075646No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA334524.896606539835794No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA306974.493337646578363No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG286434.19267909603362No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG277824.0666484183223135No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC265293.8832379198643965No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC242153.544521324946902No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAATAATCAATAATCTTGGCT193652.8345924203013317No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT164682.4105379797326276No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAGTTATGGGAAGCGGCT158782.324175494425228No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG146942.1508650154354645No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGTCA132261.935983441823156No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGAGTTATCTAACTCCCAA111441.6312263326536558No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC94771.3872157173868176No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC72531.061673060906045No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTCCACGTCGTGTCTTTCACAG66630.9753105755986458No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACAACAATCTTAA64380.9423757295068409No Hit
TCCGTAGGTGAACCTGCGGAGGGATCAATAATACAATAAAAAAATCAAAA60300.8826538752603683No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAGAAATGAGGCAAAGGGCG56920.8331784175757905No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAGAGAAATGAGGCAAAGGGCG55690.8151740350456038No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAATTAATTTATTACATTGT51320.7512072450806319No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACACTAAAACATTCTATT50150.7340811251128934No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGATTCGTATTAACCTGCC49400.7231028430822918No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCAATGTTCTT47810.6998288851774164No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAATTCAATTGCAGACGC47570.6963158349276238No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCAAAAAACTATCCA42450.6213707629320503No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCAGGTCCAAAACCT41490.6073185619328803No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGACCGAAAGG39550.5789214057470574No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCG37760.552719905967355No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC20730.3034397153258281No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGCAAGG20510.3002194192635183No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAATTTTAATGTTTGTTTT15150.22176129701815223No Hit
TCCGTAGGTGAACCTGCGGAATGATCATTTACATATCCAATCCAACCTAA14950.21883375514332512No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTGCACATATCCAAACCTTAAA14770.21619896745598075No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC12910.1889728280200888No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACAGAGTTCATGCCCGAAAGG12040.17623802086459095No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACCAATCTTGACTCGAAAGAG9400.13759446811687331No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG8960.13115387599225373No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCACATAAACCTAT8850.1295437279610988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7970.11666254371185962No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGACAGG7030.10290309690017228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)