FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683167
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT16656724.381593373216212No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG7947711.633612279281639No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG620769.086504471088329No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT414066.060889943454529No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT290924.258402411123488No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA220033.2207351935910253No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG210463.080652314880549No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA182922.6775297987168583No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG118751.738227988178586No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT113931.6676742289952529No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT109131.5974132239994026No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAATAGATCCGTTGTTGATAGTTA81871.19838926646047No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGATGAAAGTTT78801.1534514986818742No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAGAGTTT69611.0189309495335692No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCAAAAGTGC69171.0124903574089497No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGTAAGTTT68090.9966816312848835No Hit
GCTGCGTTCTTCATCGATTCGAGACCCAAGGAATCCATTGTCTCGAGTTG62880.9204191654456376No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT55630.8142957724831557No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG54480.7974624067028998No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAAATTT54450.7970232754216757No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGATATCCACCGTTGAAAATTT50830.7440347674873055No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT49970.731446337425549No Hit
GCTGCGTTCTTCATCGATGTGCGAGCCTAGACATCCACTGTTGAAAGTTG42790.6263475841192564No Hit
GCTGCGTTCTTCATCGATGTGGGAACCTAGACATCCGTTGCTGAAAGTTG41280.6042446429643118No Hit
GCTGCGTTCTTCATCGTTGTTCAAGCCGAGGCATCCATCATTGAAAATGT27220.3984384491639672No Hit
GCTGCGTTCTTCATCGATGTTCAAGCCGAGGCATCCATCATTGAAAATGT24390.35701373163516387No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA20200.2956817293575363No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT19020.27840923229605646No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT16470.24108307339201102No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT16460.24093669629826966No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAATTT15520.22717724948658233No Hit
GCTGCGTTCTTCATCGATGCATAAGCCGTGTGCTCCATTGCACAGAGTTT15410.22556710145542744No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT14130.20683083345653408No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG12850.18809456545764067No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG12240.17916556273941803No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG12230.17901918564567668No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG11590.16965105164622998No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGACATCCACCGTTGAAAATTT10740.15720899867821483No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT10030.14681622502257868No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT9360.1370089597419079No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7730.1131494934620671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)