FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861501
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACAACAATCTTAA829959.633767111123493No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC780719.062206544159555No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT710898.25176058994708No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA707608.213571429400545No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT572026.639806570160685No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG424994.933134146100817No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTTATATGCAGTC273673.1766649139118814No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG259933.0171758361278744No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC226332.6271588773547565No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCCATCCACATCCACTCG223232.5911751698489036No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGCAGAGTTCATGCCCGAAAGG178442.0712686346272378No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACGCCAATTATTCTTCTTGC164491.9093419508508984No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA164311.9072525742860427No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTTCGATGAAAAACCTTTTTTCA141651.6442232800658385No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTTAGGGTCTCTCTGG141541.6429464388317598No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACTC140711.6333120913382575No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAACAAACCTTAA136861.5886226481455041No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAACGTATCCACGTATAACCC121611.4116060225118716No Hit
TCCGTAGGTGAACCTGCATAAGGATCATTAGTGAAGATTTGGGCAGGCCA89861.04306321176644No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC50040.5808466850299652No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCACATAAACCTAA36150.41961646010857795No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC32350.37550739929495147No Hit
TCCGTAGGTGAACCTGCGGATGGATCATTAACACAATTAACAAACCTTAA30980.3596049221068809No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC27480.31897815556801445No Hit
TCCGTAGGTGAACCTGCGGACGGCATCGATGAAGAACGCAGC26850.31166533759101844No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA21710.2520020290167974No Hit
TCCGTAGGTGAACCTGCGGAGGGCATCGATGAAGAACGCAGC21410.24851973474203742No Hit
TCCGTAGGTGAACCTGCGGAAGTATCATTCACGCCAATTATTCTTCTTGC18200.2112591860021056No Hit
TCCGTAGGTGAACCTGCGGAGGTGAACCTGCATCGATGAAGAACGCAGC14580.16923950175333516No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC14470.1679626605192565No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG14330.16633758985770186No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC12870.14939042438720326No Hit
TCCGTAGGTGAACCTGCGGGGGCATCGATGAAGAACGCAGC11850.13755062385301933No Hit
TCCGTAGGTGAACCTGCGGAAGTTTCACTACCCTTTTTCCATTTGCATCGATGAAGAACGCAGC10390.12060345838252075No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGCGCGT10280.11932661714844207No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACACAAAACAACAATCTTAA8910.10342413996037149No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCCCTTTGAGCGT8740.10145083987134083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)