FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861501
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG10893912.645255199935926No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT10712112.43422816688547No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA787109.136379412211943No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAAATTT665807.728371760450655No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT631627.331622366079668No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT513345.958669810017632No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA445455.170626615639448No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA280023.2503734760609677No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAGAGTTG238212.765057730635252No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGATATCCATCGTTGAAAATTT186852.168888950796343No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT169511.9676123417152156No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCAAAAGTTG158101.8351690827985112No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGACATCCACCGTTGAAAATTT157401.8270437294907378No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCATCGTTGAGGATTT106591.237259155822222No Hit
GCTGCGTTCTTCATCGATACGAGAGCCTAGATATCCGTTGTTAAGAATTG67780.7867663531441055No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGTTAAGAATTG57260.6646539005758554No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA56530.6561803178406062No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT38520.44712658487918183No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG35510.41218756565575665No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA33310.3866507409741834No Hit
GCTGCGTTCTTCATCGATGCCGTCCGCAGGTTCACCTACGGA29720.344979286152889No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGATATCCATCGTTGAGGATTT28280.32826427363404104No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA27310.31700485547898377No Hit
GCTGCGTTCTTCATCGATGCCCTCCGCAGGTTCACCTACGGA23150.26871704153564535No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG21990.25525217033990677No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT17920.20800904467899634No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT16670.1934994852008297No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTCCGCAGGTTCACCTACGGA14540.16877519585003384No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA14360.16668581928517784No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA14290.16587328395440054No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT12240.14207760641020728No Hit
GCTGCGTTCTTCATCGATGCCCCCGCAGGTTCACCTACGGA12160.14114899460360464No Hit
GCTGCGTTCTTCATCGATGCAAATGGAAAAAGGGTAGTGAAACTTCCGCAGGTTCACCTACGGA10750.12478221151223272No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT10040.11654078172863408No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9400.1091118872758128No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT8760.1016829928229915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)