FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681001
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA9736314.297042148249414No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAGGCAGAA7960111.688822777059064No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG614809.027886890033935No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA405455.953735750755138No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCTTCAGTGGTTTTCCACTG399675.86886069183452No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCAATGTTCTT302224.4378789458458945No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA285844.197350664683311No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAC236163.4678363174209728No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA223293.2788498107932296No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAATAACCTTCGGGTTA182132.6744454119744314No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG173112.5419933304062696No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC166392.4433150612113637No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACGCGATCCAAATCACGC144602.123344899640382No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACCGATTTAGAAAGGACGAAC97491.4315691166385953No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCTCTTGTTTTTTTTGTTGTGT83021.2190877840120646No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC50770.7455201974740125No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG39280.5767979782702228No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATATTTAAAAGGTAAAAGAAAATA36240.5321578088725273No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTTCTACTCCCAAA16150.2371508999252571No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGAAACCTGCAAGGCAGAA8980.1318647109181925No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8130.11938308460633686No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCACGGCAGAA7600.11160042349423864No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA7220.1060204023195267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)