FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809499
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA13922917.199403581721533No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACAACAATCTTAA13700616.924789283248035No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG707248.73676187370213No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG662418.182962548440456No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA598437.392597149594997No Hit
TCCGTAGGTGAACCTGCGTAAGGATCATTAACGTGATGTATCTATCACAT517186.388889918332202No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG387114.782093615927876No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC242532.9960506436697267No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC144201.7813487107457824No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTGCAAGTGACCCCGGTCTAACC45840.5662761782287563No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG38000.4694261512367526No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC32070.3961709650042804No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC27130.3351455653435026No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA17400.21494776398735516No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACACAAAACAACAATCTTAA14620.18060553502845586No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC13000.16059315700204696No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACAACACTCTTAA12800.1581224930481693No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACTGATTCTGCCTGGCGTGTC12530.15478709671043447No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC11030.13625711705635213No Hit
TCCGTAGGTGAACCTGCGTAAGGATCATTAACGGGATGTATCTATCACAT9930.1226684653100251No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA9800.12106253374000461No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGCTTTGGGCAGGCCA9610.11871540298382087No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA9600.11859186978612699No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT9360.11562707304147381No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGCGAAGATTTGGGCAGGCCA9000.11117987792449405No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTTGGGCAGGCCA8370.10339728646977944No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAAACCACAATCTTAA8370.10339728646977944No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAAAACAACAATCTTAA8100.10006189013204464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)