FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809499
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG17415021.513306378389597No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAAATTT11318213.981734381388982No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT10708413.22842894185169No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA751469.283025673904477No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT703118.685742663054556No Hit
GCTGCGTTCTTCATCGATCCGCGAGCCGAGTGATCCACCGCTCAGAGTGT281703.4799301790366632No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGACATCCACCGTTGAAAATTT248823.0737530250191787No Hit
GCTGCGTTCTTCATCGATGCGCGAGCCGAGTGATCCACCGCTCAGAGTGT247063.0520111822250553No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT203362.51217110830279No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG47690.5891298198021245No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC32930.4067948200059543No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA27220.33625736412274754No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT14150.1747994747368434No Hit
GCTGCGTTCTTCATCGATGCATAAGCCGTGTGCTCCATTGCACAGAGTTT13760.169981680026782No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG13690.16911694764292481No Hit
GCTGCGTTCTTCATCGGTGTAGGAACCTAGACATCCACCGTTGAAAATTT13490.16664628368904716No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT12450.15379883112888343No Hit
GCTGCGTTCTTCATCGTTGTCGGAACCTAGACATCCACCGTTGAAAATTT12260.15145170037269964No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA12240.15120463397731188No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT11240.13885131420792368No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG10800.13341585350939283No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10680.13193345513706625No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG9960.12303906490310675No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTCGCGGTGGGGGGGTGGGTG9830.12143313333308627No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG9660.11933306897229028No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9600.11859186978612699No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC9420.1163682722276371No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAACTTT9070.11204461030835122No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG8840.10920334676139193No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGACATCCACCGTTGAAAGTTT8780.10846214757522864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)