FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779383
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAAGCAGAA9694512.438685472995948No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA9482812.167060354151939No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCCATCCACATCCACTCG559027.172596784892665No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATTATCAATACCACAACGT480836.169367307216093No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACACATCCAATCAAACCTAA469866.028614942845816No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA444215.6995084573309915No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTAT303793.897826870742626No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA284633.6519913829272643No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCACTTTTGGTTTTTAGGCC173652.228044491604256No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAACAAACCTTAA153561.9702764879398191No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACTGATTTTGTCATGTTGGGT149551.9188255325045582No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCAAGATTGCGAGCCTCGCA149321.9158744801977976No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCACATAAACCTAA135841.7429171536972197No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGTACCGTTTTCGGGGGAT119381.5317244538307866No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTTTGTGGGTTTTTGTATGGTA113921.4616690382007307No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC94441.2117277384803107No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCAATAATCCTAAACAAGGA86821.1139580924911117No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCCAATAATTCTTCTTGC83341.0693073880236033No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC59290.7607299620340705No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAATTTTAATGTTTGTTTT55650.7140263516140332No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG33360.4280308911023207No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCACGCCAATTATTCTTCTTGC30880.39621084883811936No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGA17840.22889901370699642No Hit
TCCGTAGGTGAACCTGCCCATTGTGCATCGATGAAGAACGCAGC17780.22812917397479804No Hit
TCCGTAGGTGAACCTCGGTGGTCAGGATTCAGAGGGGCTTTTGGTTTCCC14550.1866861350581165No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAACGCAGAA11570.1484507616922617No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTGTCGAAACCTGCAAAGCAGAA10610.13613332597708702No Hit
TCCGTAGGTGAACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCACC10450.1340804200245579No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACTGAGTGTAAAAACTCCCAA9940.12753678230087134No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCCATCCACATCCCCTCG7970.1022603777603566No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATGATCAATACCACAACGT7900.1013622314061251No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAGTGTAAAAACTCCCAA7850.10072069829595975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)