FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002462842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779383
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAGAGTTG11091214.230744062931834No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT10158413.033899892607357No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA9812312.589830673750905No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT470166.032464141506807No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT467385.996794900581614No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTAAAAATTT322884.1427642122037565No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG309043.9651878473099873No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG171012.194171543387526No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT161472.071767025967977No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGTGATCCATTGCTGGCAGTTT152501.9566759860043137No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT129181.657464943423195No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG100601.2907646176526817No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCATTGCTAAGAGTTG100331.2873003388577888No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGGTCCACCGTTAGAAGTTG94431.2115994318582777No Hit
GCTGCGTTCTTCATCGATACAAGAGCCAAGTCGTCCATTATGAAGACTTG85331.0948404058081842No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGATATCCATTGCTAAGAGTTG81961.0516010741830397No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCACCGTTTAAAATTT70850.9090524171042992No Hit
GCTGCGTTCTTCATCGATCCACGAGCCAAGAGATCCACCGTTAAAAGTTG70040.8986595807196206No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT59420.7623979481205004No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA58730.7535447912002187No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGTCGTCCATTATGAAGACTTG36280.4654964247359771No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGATATCCATCGTTGAAAATTT31500.4041658594041697No Hit
GCTGCGTTCTTCATCGATGCACGAGCCAAGAGATCCACCGTTAAAAGTTG28740.3687532317230425No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT25140.32256284779113736No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGGGGTCCACCGTTAGAAGTTG24710.31704566304371534No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT24650.31627582331151693No Hit
GCTGCGTTCTTCATCGATGCACAATGGGCAGGTTCACCTACGGA18310.23492942494255073No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAGAGTTG15230.19541098535636522No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCAATCGCTGAGGTTTT15200.19502606549026602No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAGAGTTT15160.19451283900213376No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT13550.1738554728548095No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTCGAGAGTTG13450.17257240663447881No Hit
GCTGCGTTCTTCCCAGCACAGACAAGGAAAGCATCGCCAGGGCGGAGCTG12300.1578171451006758No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG10870.13946929814994682No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCACCGTTGAAAATTT10550.1353634862448886No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGGCA10140.13010291474153274No Hit
GCTGCGTTCTTCATCGATGCAGCAGACGCCTGGAACTTCACAGGGTTTCT9710.12458572999411073No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGAGATCCGTTGCCGAGAGTCA8490.10893232210607623No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCTGAGAGTTG8140.10444159033491877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)