FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745017
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA24800033.28783101593655No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG7536910.116413450968233No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA677729.096705175855048No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT596328.00411265783197No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATAGTGATTTGCCTTCGGGCTAAA524787.043866113122251No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC270933.6365613133660037No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA188782.5339019109631056No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGGTGACCCCGGTTTACCA74500.9999771817287392No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG47960.6437436998081923No Hit
TCCGTAGGTGAACCTGCGGCAACCCCCTCTCCTCTTGGGGGGGGCGGGGG47480.6373008938051078No Hit
TCCGTAGGTGAACCTGCGGCGTGTTTGCTCCCCTCTGGGCTGTGAGACCC33130.4446878393378943No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACTGAGTTTTTAACTCTCCAA26060.34979067591746227No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCCCCAA23530.31583171927620446No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAGTTTTTAACTCTCCAA17780.23865227236425476No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCCA17330.23261214173636308No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATAGTGAGTTGCCTTCGGGCTAAA17300.23220946636117026No Hit
TCCGTAGGTGAACCCGAGGAAGGCCCCCCAGGCCCTGTGGGCATCGGTGA13850.1859017982140005No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA12350.16576802945436145No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC11240.15086904057222855No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGGTTTTAACTCTCCAA10040.1347620255645173No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTTACTATACTTTTG8500.11409135630462125No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAC7960.1068431995511512No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTATTGATTTGACTATACTTTTG7960.1068431995511512No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTTCCTTCGGGCTAA7880.10576939855063709No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGATTTTAACTCTCCAA7880.10576939855063709No Hit
TCCGTAGGTGAACCTGCGGAGGGAGCATTACTGAGTTTTTAACTCTCCAA7590.10187686992377357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)