FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745017
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT22802530.60668414277795No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA13507918.130995668555215No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA7578710.172519553245094No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT677699.096302500479855No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT492556.6112585350401405No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT295593.9675604717744695No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCTGTTGTTGAAAGTTT169742.278337272840754No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG69770.936488697573344No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA64930.871523737042242No Hit
GCTGCGTTCTTCATCGAGGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT35400.47515694272748144No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA34770.46670075984843296No Hit
GCTGCGTTCTTCATCGAGGCCAGAACCAAGAGATCTGTTGTTGAAAGTTT25070.3365023885361005No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT23580.31650284490152575No Hit
GCTGCGTTCTTCATCGATGCCCACAGGGCCTGGGGGGCCTTCCTCGGGTT14190.19046545246618535No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGGTT14190.19046545246618535No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG12690.1703316837065463No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT12400.16643915507968274No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA12330.16549957920423294No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA11700.15704339632518452No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC11680.15677494607505602No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGATAGTTT9740.1307352718125895No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG9000.12080261255783425No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGGTA8930.11986303668238443No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTA8740.11731275930616349No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT8470.11368868092942845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)