FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696133
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA13057318.75690421226978No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT11017215.826286068897755No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT9865414.171717186227346No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG460256.611523947291681No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG406035.832649795369563No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG367265.275715991053434No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA275353.955422311541042No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG166882.3972430555655313No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTT137851.9802250432029513No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG132331.9009298510485784No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAGAGTTT127941.837867189172184No Hit
GCTGCGTTCTTCATCGATGCACACATGAGGGCCACGTCAGGTGACCACTC98531.4153904498134695No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT90321.2974532165548824No Hit
GCTGCGTTCTTCATCGATGCACACATGGGGGCCACAGCAGGGGACCGCTC71781.0311247994276955No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTA69981.0052676715512696No Hit
GCTGCGTTCTTCATCGATGCGCGTTCACCTACGGA18070.2595768337372312No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT17810.25584191526619193No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT14400.20685702301140732No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA14280.20513321448631222No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA13630.195795918308714No Hit
GCTGCGTTCTTCATCGATGCCCGAAACCAGAACAACTACCACTGCCACTG12490.1794197373203109No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG9840.141352299057795No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTA9700.13934118911185076No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG8930.12828008440915745No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG7530.10816898494971507No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT7420.10658882713504458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)