FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756460
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC20321026.86328424503609No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA9684812.802791951986887No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG622018.222642307590618No Hit
TCCGTAGGTGAACCTGCGGAGGGATTATTAATGAGAACGTGCCCTCACGG584337.724532691748406No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT543757.188086613965047No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCTTAGGGGAAATC230173.0427253258599265No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG138861.8356555534991936No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCG137921.823229252042408No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT118751.569812019141792No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC54790.7242947412949793No Hit
TCCGTAGGTGAACCTGCGGAGGGATGATTAATGAGAACGTGCCCTCACGG35970.4755043227665706No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC23600.31197948338312664No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACTGATTTGCTTAATTGCACC23310.308145837189012No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC22350.29545514633952885No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTTCTTAATTGCACC22160.292943447108902No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG22080.29188588953811173No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA22020.291092721360019No Hit
TCCGTAGGTGAACCTGCGGCCTGCGTGGTGCTGAGACTGCCTCTGGCCGC18670.2468074980831769No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTTCACC15530.20529836342965924No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTACTTGCACC14650.19366523015096634No Hit
TCCGTAGGTGAACCTGGCCTTCCATGATCAATGGCTGTCGAAGCCTTGGG13690.18097453930148322No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCCCC13000.17185310525341724No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGATTTGCTTAATTGCACC12250.16193850302725854No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACAAGTGACCCCGGTTTACCA11030.14581075007270708No Hit
TCCGTAGGTGAACCATCTGGAGATCGATGAAGAACGCAGC9530.1259815456203897No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGTGACCCCGGTTTACCA8950.11831425323216033No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8680.11474499643074319No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC7890.10430161541918938No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAGAACGTGCCCTCACGG7600.10046796922507469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)