FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756460
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT24506432.39616106601803No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT13250817.51685482378447No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT12276816.22927848134733No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAGAGTTT483256.388308701054914No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG255223.3738730402136263No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC119121.574703222906697No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG57590.7613092562726383No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA55170.7293181397562329No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT30540.4037226026491817No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA24610.3253311477143537No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG22230.29386880998334347No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA21330.28197128731195303No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT21120.27919519868862863No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAATGTTT19470.2573830737910795No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGATAGTTT13490.17833064537450757No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCTGAAAGTTT13300.17581894614388072No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG11940.15784046744044627No Hit
GCTGCGTTCTTCATCCAGAAGCCCCCGGGCAGTAAGCAGCCACTTCAGGC11470.1516273167120535No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT11080.146471723554451No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGGTT10620.140390767522407No Hit
GCTGCGTTCTTCATCGATCTCCAGATGGTTCACCTACGGA9420.1245274039605531No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTT9190.12148692594453112No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG9170.12122253655183354No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT8530.11276207598551145No Hit
GCTGCGTTCTTCATCGCACTTGCGGCCCCGGGCAGCCGGGGCCGCAAGTG8270.1093250138804431No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8260.10919281918409433No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC7980.10549136768632841No Hit
GCTGCGTTCTTCACCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT7740.10231869497395764No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTCGTTGAAAGTTT7730.10218650027760887No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG7620.10073235861777226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)