FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760401
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG7656210.068634838723254No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA664938.744465091445171No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCAATGTTCTT646428.501040898157683No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA635088.351909058509918No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG611098.03641762701522No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCATGTTCTTC588717.742099234482859No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAGTTAATACACTAATA519676.834157240719042No Hit
TCCGTAGGTGAACCTGCGGACGGATCATTATTGCTAACCACAACAGTAAT388495.109014848744281No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCTATCAATACAAAAACC319094.1963385108646625No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA273173.5924466169823552No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC200742.639922882794736No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA130671.7184354044773742No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAACAAACCTTAA64670.8504723165803305No Hit
TCCGTAGGTGAACCTGCGGCCGATTTCCGGGTGGAGGGGCCGGCCGAGCA40950.538531643172484No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACATCCACATAAACCTAA36930.48566480054602773No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG30300.3984739630800065No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGAGGGCCCTCTGGG8430.1108625580450315No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG8140.10704878084063539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)